ArticleInvestigative ophthalmology & visual science2012
Effect of the synthetic NC-1059 peptide on diffusion of riboflavin across an intact corneal epithelium.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- Changes in the Biomechanical Properties of Corneal Stromal Lens after Collagen Crosslinking Induced by EDC-NHS.Journal of ophthalmology · 2024Article
- Biomaterial composed of chitosan, riboflavin, and hydroxyapatite for bone tissue regeneration.Scientific reports · 2023Article
- Development of 3D DLP Printed Sustained Release Ibuprofen Tablets and Their Pharmacokinetic Evaluation in Rats.AAPS PharmSciTech · 2023Article
- In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy.Scientific reports · 2016Article
- Corneal stromal demarcation line after collagen cross-linking in corneal ectatic diseases: a review of the literature.Clinical ophthalmology (Auckland, N.Z.) · 2016Review
- Anti-Apoptotic Gene Delivery with cyclo-(d-Trp-Tyr) Peptide Nanotube via Eye Drop Following Corneal Epithelial Debridement.Pharmaceutics · 2015Article
- Riboflavin concentration in corneal stroma after intracameral injection.International journal of ophthalmology · 2015Article
- Imaging mass spectrometry by matrix-assisted laser desorption/ionization and stress-strain measurements in iontophoresis transepithelial corneal collagen cross-linking.BioMed research international · 2014Article
- Ultrasound-enhanced penetration of topical riboflavin into the corneal stroma.Investigative ophthalmology & visual science · 2013Article
- New clinical pathways for keratoconus.Eye (London, England) · 2013Review
- Nonlinear optical collagen cross-linking and mechanical stiffening: a possible photodynamic therapeutic approach to treating corneal ectasia.Journal of biomedical optics · 2013Article
- Corneal absorption of a new riboflavin-nanostructured system for transepithelial collagen cross-linking.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo investigate the effect of the peptide NC-1059 on riboflavin (RF) diffusion across an intact corneal epithelium into the stroma.
methodsNC-1059 peptide was synthesized by solid-phase synthesis with 9-fluorenylmethoxycarbonyl chemistry, characterized by reversed-phase HPLC, and matrix-assisted laser desorption ionization time-of-flight mass spectroscopy. The diffusion of RF across embryonic day 18 chick corneal epithelium ex vivo was monitored using confocal microscopy. The depth distributions of RF in the corneal stroma were calculated using a group of linear equations based on the relationship between RF fluorescence intensity and concentration.
resultsData presented in this study demonstrate that the NC-1059 peptide can transiently open the intact epithelial barrier to allow the permeation of RF into the stroma. The effect of NC-1059 peptide on RF diffusion across the corneal epithelium was concentration and time dependent. The amount of RF reaching a 50-μm depth of chick corneal stoma increased dramatically after exposure to NC-1059 for 10 minutes, reaching a plateau by 30 minutes. The concentrations of RF in the presence of NC-1059 at corneal stromal depths of 50, 100, and 150 μm were significantly higher than in the absence of the peptide, and almost as high as in corneas in which the epithelium first had been physically removed. In addition, a cell viability assay indicated that the NC-1059 peptide did not kill corneal epithelial cells.
conclusionsNC-1059 peptide significantly enhances the diffusion of RF across intact corneal epithelium into the stroma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.