ArticleThe Plant cell2012
A previously unknown oxalyl-CoA synthetase is important for oxalate catabolism in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 76 citations in OpenAlex.
- Gap Analysis of Metabolic Conversions of Off-Flavors and Antinutrients in Plant-Based Substrates.Comprehensive reviews in food science and food safety · 2026Review
- Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.Plant physiology · 2026Review
- Circular cell clusters and calcium oxalate crystals: critical players in Solanaceae anther dehiscence.Planta · 2025Review
- Genome editing of an oxalyl-CoA synthetase gene in Lathyrus sativus reveals its role in oxalate metabolism.Plant cell reports · 2024Article
- Optimization of Plant Oxalate Quantification and Generation of Low-Oxalate Maize (Plants (Basel, Switzerland) · 2024Article
- Nitrogen reduces calcium availability by promoting oxalate biosynthesis in apple leaves.Horticulture research · 2024Article
- LsBOS utilizes oxalyl-CoA produced by LsAAE3 to synthesize β-ODAP in grass pea.Nature communications · 2024Article
- Disruption of theInternational journal of molecular sciences · 2024Article
- Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit.Molecular horticulture · 2024Article
- Identification and characterization of Glycolate oxidase gene family in garden lettuce (Lactuca sativa cv. 'Salinas') and its response under various biotic, abiotic, and developmental stresses.Scientific reports · 2023Article
- Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.Nature communications · 2023Article
- A conserved oxalyl-coenzyme A decarboxylase in oxalate catabolism.Plant signaling & behavior · 2022Article
- Oxalic acid degradation in wood-rotting fungi. Searching for a new source of oxalate oxidase.World journal of microbiology & biotechnology · 2022Article
- Biosynthesis of plant hemostatic dencichine in Escherichia coli.Nature communications · 2022Article
- Maize RNA 3'-terminal phosphate cyclase-like protein promotes 18S pre-rRNA cleavage and is important for kernel development.The Plant cell · 2022Article
- The identification and characterization of an oxalyl-CoA synthetase from grass pea (RSC chemical biology · 2022Article
- Pathogen-induced expression of a blight tolerance transgene in American chestnut.Molecular plant pathology · 2022Article
- Digital gene expression profiling of the transcriptional response toFrontiers in microbiology · 2022Article
- Tomato Oxalyl-CoA Synthetase Degrades Oxalate and Affects Fruit Quality.Frontiers in plant science · 2022Article
- The Tomato Transcription Factor SlNAC063 Is Required for Aluminum Tolerance by RegulatingFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxalate is produced by several catabolic pathways in plants. The best characterized pathway for subsequent oxalate degradation is via oxalate oxidase, but some species, such as Arabidopsis thaliana, have no oxalate oxidase activity. Previously, an alternative pathway was proposed in which oxalyl-CoA synthetase (EC 6.2.1.8) catalyzes the first step, but no gene encoding this function has been found. Here, we identify acyl-activating enzyme3 (AAE3; At3g48990) from Arabidopsis as a gene encoding oxalyl-CoA synthetase. Recombinant AAE3 protein has high activity against oxalate, with K(m) = 149.0 ± 12.7 μM and V(max) = 11.4 ± 1.0 μmol/min/mg protein, but no detectable activity against other organic acids tested. Allelic aae3 mutants lacked oxalyl-CoA synthetase activity and were unable to degrade oxalate into CO(2). Seeds of mutants accumulated oxalate to levels threefold higher than the wild type, resulting in the formation of oxalate crystals. Crystal formation was associated with seed coat defects and substantially reduced germination of mutant seeds. Leaves of mutants were damaged by exogenous oxalate and more susceptible than the wild type to infection by the fungus Sclerotinia sclerotiorum, which produces oxalate as a phytotoxin to aid infection. Our results demonstrate that, in Arabidopsis, oxalyl-CoA synthetase encoded by AAE3 is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen.
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