ArticleThe Plant cell2012
Sulfamethazine suppresses epigenetic silencing in Arabidopsis by impairing folate synthesis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 89 citations in OpenAlex.
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- The Association Between DNA Methylation and Three-Dimensional Genome During Whole Genome Doubling inPlants (Basel, Switzerland) · 2025Article
- High-Throughput Screening Identification of Chemical Compounds That Affect Cold-Regulated Gene Expression inBio-protocol · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Grapevine cell response to carbon deficiency requires transcriptome and methylome reprogramming.Horticulture research · 2025Article
- Exploring Cereal Metagenomics: Unravelling Microbial Communities for Improved Food Security.Microorganisms · 2024Review
- DNA methylome analysis reveals novel insights into active hypomethylated regulatory mechanisms of temperature-dependent flower opening inHorticulture research · 2024Article
- Chemical screening approach using single leaves identifies compounds that affect cold signaling in Arabidopsis.Plant physiology · 2023Article
- Exploring the crop epigenome: a comparison of DNA methylation profiling techniques.Frontiers in plant science · 2023Review
- Redox status of the plant cell determines epigenetic modifications under abiotic stress conditions and during developmental processes.Journal of advanced research · 2022Review
- Metabolic network changes during skotomorphogenesis inPlant direct · 2022Article
- Dysfunction of histone demethylase IBM1 in Arabidopsis causes autoimmunity and reshapes the root microbiome.The ISME journal · 2022Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- The 5-formyl-tetrahydrofolate proteome links folates with C/N metabolism and reveals feedback regulation of folate biosynthesis.The Plant cell · 2021Article
- Genome-wide shifts in histone modifications at early stage of rice infection with Meloidogyne graminicola.Molecular plant pathology · 2021Article
- Nicotinamide Effectively Suppresses Fusarium Head Blight in Wheat Plants.International journal of molecular sciences · 2021Article
- Dicer-like proteins influence Arabidopsis root microbiota independent of RNA-directed DNA methylation.Microbiome · 2021Article
- Screening of Emerging Pollutants (EPs) in Estuarine Water and Phytoremediation Capacity ofInternational journal of environmental research and public health · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
DNA methylation is a critical, dynamically regulated epigenetic mark. Small chemicals can be valuable tools in probing cellular processes, but the set of chemicals with broad effects on epigenetic regulation is very limited. Using the Arabidopsis thaliana repressor of silencing1 mutant, in which transgenes are transcriptionally silenced, we performed chemical genetic screens and found sulfamethazine (SMZ) as a chemical suppressor of epigenetic silencing. SMZ treatment released the silencing of transgenes as well as endogenous transposons and other repetitive elements. Plants treated with SMZ exhibit substantially reduced levels of DNA methylation and histone H3 Lys-9 dimethylation, but heterochromatic siRNA levels were not affected. SMZ is a structural analog and competitive antagonist to p-aminobenzoic acid (PABA), which is a precursor of folates. SMZ decreased the plant folate pool size and caused methyl deficiency, as demonstrated by reductions in S-adenosylmethionine levels and in global DNA methylation. Exogenous application of PABA or compounds downstream in the folate biosynthesis pathway restored transcriptional silencing in SMZ-treated plants. Together, our results revealed a novel type of chemical suppressor of epigenetic silencing, which may serve as a valuable tool for studying the roles and mechanisms of epigenetic regulation and underscores an important linkage between primary metabolism and epigenetic gene regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.