ArticleApplied and environmental microbiology2012
Stability of and attachment to lettuce by a culturable porcine sapovirus surrogate for human caliciviruses.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Prescribed RNA Particle Vaccine Against Porcine Sapovirus Enhances Virus-Neutralizing Antibody Titers in Colostrum and Milk.Pathogens (Basel, Switzerland) · 2026Article
- Impact of irrigation water quality on human norovirus surrogate survival during leafy green production.Frontiers in plant science · 2023Article
- The Basis of Peracetic Acid Inactivation Mechanisms for Rotavirus and Tulane Virus under Conditions Relevant for Vegetable Sanitation.Applied and environmental microbiology · 2020Article
- Porcine sapoviruses: Pathogenesis, epidemiology, genetic diversity, and diagnosis.Virus research · 2020Review
- Tissue Distribution and Visualization of Internalized Human Norovirus in Leafy Greens.Applied and environmental microbiology · 2018Article
- Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process.Food and environmental virology · 2018Article
- Article
- Antiviral effect of theaflavins against caliciviruses.The Journal of antibiotics · 2017Article
- A Bacterial Surface Display System Expressing Cleavable Capsid Proteins of Human Norovirus: A Novel System to Discover Candidate Receptors.Frontiers in microbiology · 2017Article
- Bacterial Surface-Displayed GII.4 Human Norovirus Capsid Proteins Bound to HBGA-Like Molecules in Romaine Lettuce.Frontiers in microbiology · 2017Article
- Recognition of Histo-Blood Group Antigen-Like Carbohydrates in Lettuce by Human GII.4 Norovirus.Applied and environmental microbiology · 2016Article
- Mechanism of Cell Culture Adaptation of an Enteric Calicivirus, the Porcine Sapovirus Cowden Strain.Journal of virology · 2016Article
- Abiotic Stress and Phyllosphere Bacteria Influence the Survival of Human Norovirus and Its Surrogates on Preharvest Leafy Greens.Applied and environmental microbiology · 2016Article
- Feline Calicivirus, Murine Norovirus, Porcine Sapovirus, and Tulane Virus Survival on Postharvest Lettuce.Applied and environmental microbiology · 2015Article
- Effect of temperature and relative humidity on the survival of foodborne viruses during food storage.Applied and environmental microbiology · 2015Article
- Comparison of methods for evaluating the thermal stability of human enteric viruses.Food and environmental virology · 2015Review
- Comprehensive review of human sapoviruses.Clinical microbiology reviews · 2015Review
- Comprehensive comparison of cultivable norovirus surrogates in response to different inactivation and disinfection treatments.Applied and environmental microbiology · 2014Article
- Mechanisms of antiviral action of plant antimicrobials against murine norovirus.Applied and environmental microbiology · 2014Article
- Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures.Archives of virology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human noroviruses (HuNoVs) are the leading cause of food-borne illness, accounting for 58% of U.S. cases. Because HuNoVs are unculturable, surrogates are needed to investigate transmission routes and evaluate disinfection methods. However, the current surrogates, feline calicivirus (FCV) and murine NoV (MNV), are less tolerant than HuNoVs to acid and chlorine, respectively. Porcine sapovirus (SaV) is the only culturable enteropathogenic calicivirus. In this study, the resistance of SaV to physicochemical treatments was compared to that of HuNoVs (by reverse transcription-PCR), FCV, and MNV (by infectivity assays). Sapovirus and HuNoV (viral RNA) showed similar resistances to heat (56°C) and to different concentrations of chlorine. However, SaV was more resistant than HuNoVs to ethanol treatment (60% and 70%). Like HuNoVs, SaV was stable at pH 3.0 to 8.0, with a <1.0 log(10) 50% tissue culture infective dose (TCID(50)) reduction at pH 3.0 compared to the value for pH 4.0 to 8.0. SaV and MNV showed similar resistances, and both were more resistant than FCV to heat inactivation (56°C). FCV was more resistant than MNV and SaV to ethanol, and all three viruses showed similar resistances to treatment with low concentrations of chlorine for 1 min. Those results indicate that SaV is a promising surrogate for HuNoVs. Next, we used SaV as a surrogate to examine virus attachment to lettuce at different pHs. Sapovirus attached to lettuce leaves significantly at its capsid isoelectric point (pH 5.0), and the attached viral particles remained infectious on lettuce after 1 week of storage at 4°C. The culturable SaV is a good surrogate for studying HuNoV contamination and transmission in leafy greens and potential disinfectants.
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