ArticleApplied and environmental microbiology2012
Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Biological protection of grapevine pruning wounds: Training system design and mycobiome dynamics.Pest management science · 2026Article
- Resisting Xylella fastidiosa: xylem anatomical changes in the susceptible olive cultivar Cellina di Nardò after long-term infection.Plant biology (Stuttgart, Germany) · 2026Article
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- Article
- Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease.International journal of molecular sciences · 2022Article
- Genera of phytopathogenic fungi: GOPHY 4.Studies in mycology · 2022Article
- ThePathogens (Basel, Switzerland) · 2019Article
- Molecular Effects ofPlants (Basel, Switzerland) · 2019Article
- Xylem cavitation susceptibility and refilling mechanisms in olive trees infected by Xylella fastidiosa.Scientific reports · 2019Article
- Profiling grapevine trunk pathogens in planta: a case for community-targeted DNA metabarcoding.BMC microbiology · 2018Article
- Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata.Journal of fungi (Basel, Switzerland) · 2017Article
- Development of SCAR Primers for PCR Assay to Detect Diplodia seriata.International scholarly research notices · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification of P. chlamydospora and P. aleophilum. Specificity was demonstrated against purified DNA from 60 P. chlamydospora isolates or 61 P. aleophilum isolates, and no amplification was obtained with 54 nontarget DNAs. The limits of detection (i.e., DNA detectable in 95% of reactions) were around 100 fg for P. chlamydospora and 50 fg for P. aleophilum. Detection was specific and sensitive for P. chlamydospora and P. aleophilum. Spores of P. chlamydospora and P. aleophilum were detected without the need for DNA purification. The established protocols detected these fungi in wood samples after DNA purification. P. chlamydospora was detectable without DNA purification and isolation in 67% of reactions. The detection of these pathogens in wood samples has great potential for use in pathogen-free certification schemes.
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