ArticleApplied and environmental microbiology2012
Genetic analysis of the Cronobacter sakazakii O4 to O7 O-antigen gene clusters and development of a PCR assay for identification of all C. sakazakii O serotypes.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Characterization and the host specificity of Pet-CM3-4, a new phage infecting Cronobacter and Enterobacter strains.Virus research · 2023Article
- The Detection of Foodborne Pathogenic Bacteria in Seafood Using a Multiplex Polymerase Chain Reaction System.Foods (Basel, Switzerland) · 2022Article
- Article
- Whole Genome Sequencing-Based Comparison of Food Isolates ofFrontiers in microbiology · 2019Article
- Prevalence, Antibiotic Susceptibility, and Molecular Characterization ofFrontiers in microbiology · 2019Article
- Prevalence and Molecular and Antimicrobial Characteristics ofFrontiers in microbiology · 2018Article
- Molecular Surveillance of Cronobacter spp. Isolated from a Wide Variety of Foods from 44 Different Countries by Sequence Typing of 16S rRNA, rpoB and O-Antigen Genes.Foods (Basel, Switzerland) · 2017Article
- Multiplexed Lateral Flow Test for Detection and Differentiation ofFrontiers in microbiology · 2017Article
- Prevalence, Molecular Characterization, and Antibiotic Susceptibility ofFrontiers in microbiology · 2017Article
- Genetic Diversity, Antimicrobial Susceptibility, and Biofilm Formation ofFrontiers in microbiology · 2017Article
- Chemoenzymatic synthesis of the bacterial polysaccharide repeating unit undecaprenyl pyrophosphate and its analogs.Nature protocols · 2016Article
- Bacterial β-Kdo glycosyltransferases represent a new glycosyltransferase family (GT99).Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Simultaneous Rapid Detection and Serotyping of Cronobacter sakazakii Serotypes O1, O2, and O3 by Using Specific Monoclonal Antibodies.Applied and environmental microbiology · 2016Article
- Article
- The speciation and genotyping of Cronobacter isolates from hospitalised patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Genotyping and Source Tracking of Cronobacter sakazakii and C. malonaticus Isolates from Powdered Infant Formula and an Infant Formula Production Factory in China.Applied and environmental microbiology · 2015Article
- Diversity of O Antigens within the Genus Cronobacter: from Disorder to Order.Applied and environmental microbiology · 2015Article
- A Cronobacter turicensis O1 antigen-specific monoclonal antibody inhibits bacterial motility and entry into epithelial cells.Infection and immunity · 2015Article
- The Membrane Proteins Involved in Virulence of Cronobacter sakazakii Virulent G362 and Attenuated L3101 Isolates.Frontiers in microbiology · 2015Article
- Strategies for the identification and tracking of cronobacter species: an opportunistic pathogen of concern to neonatal health.Frontiers in pediatrics · 2015Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-negative bacterium Cronobacter sakazakii is an emerging food-borne pathogen that causes severe invasive infections in neonates. Variation in the O-antigen lipopolysaccharide in the outer membrane provides the basis for Gram-negative bacteria serotyping. The O-antigen serotyping scheme for C. sakazakii, which includes seven serotypes (O1 to O7), has been recently established, and the O-antigen gene clusters and specific primers for three C. sakazakii serotypes (O1, O2, and O3) have been characterized. In this study, the C. sakazakii O4, O5, O6, and O7 O-antigen gene clusters were sequenced, and gene functions were predicted on the basis of homology. C. sakazakii O4 shared a similar O-antigen gene cluster with Escherichia coli O103. The general features and anomalies of all seven C. sakazakii O-antigen gene clusters were evaluated and the relationship between O-antigen structures and their gene clusters were investigated. Serotype-specific genes for O4 to O7 were identified, and a molecular serotyping method for all C. sakazakii O serotypes, a multiplex PCR assay, was developed by screening against 136 strains of C. sakazakii and closely related species. The sensitivity of PCR-based serotyping method was determined to be 0.01 ng of genomic DNA and 10(3) CFU of each strain/ml. This study completes the elucidation of C. sakazakii O-antigen genetics and provides a molecular method suitable for the identification of C. sakazakii O1 to O7 strains.
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