ArticleApplied and environmental microbiology2012
Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 90 citations in OpenAlex.
- Engineered Microbial Strains for Co-fermentation of C5 and C6 Sugars to Produce Cellulosic Ethanol.Advances in biochemical engineering/biotechnology · 2026Review
- Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex.Microbiology spectrum · 2025Article
- An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation.Bioresources and bioprocessing · 2022Article
- Review
- Xylo-Oligosaccharide Utilization by EngineeredFrontiers in bioengineering and biotechnology · 2022Review
- Disruption of theFrontiers in microbiology · 2021Article
- EngineeredBioengineered · 2020Review
- Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrialBiotechnology for biofuels · 2020Article
- Secretion of Acetylxylan Esterase FromFrontiers in bioengineering and biotechnology · 2019Article
- Elucidating structure-performance relationships in whole-cell cooperative enzyme catalysis.Nature catalysis · 2019Article
- Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-AccumulatingFrontiers in microbiology · 2018Article
- Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts.Frontiers in microbiology · 2018Review
- Enhanced production of xylanase by3 Biotech · 2017Article
- Harnessing yeast organelles for metabolic engineering.Nature chemical biology · 2017Review
- Engineering of Saccharomyces cerevisiae to utilize xylan as a sole carbohydrate source by co-expression of an endoxylanase, xylosidase and a bacterial xylose isomerase.Journal of industrial microbiology & biotechnology · 2016Article
- Protein design for pathway engineering.Journal of structural biology · 2014Review
- Article
- Molecular tools for chemical biotechnology.Current opinion in biotechnology · 2013Review
- Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation.Microbial biotechnology · 2013Article
- Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum.Biotechnology for biofuels · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabinoxylan is a heteropolymeric chain of a β-1,4-linked xylose backbone substituted with arabinose residues, representing a principal component of plant cell walls. Here we developed recombinant Saccharomyces cerevisiae strains as whole-cell biocatalysts capable of combining hemicellulase production, xylan hydrolysis, and hydrolysate fermentation into a single step. These strains displayed a series of uni-, bi-, and trifunctional minihemicellulosomes that consisted of a miniscaffoldin (CipA3/CipA1) and up to three chimeric enzymes. The miniscaffoldin derived from Clostridium thermocellum contained one or three cohesin modules and was tethered to the cell surface through the S. cerevisiae a-agglutinin adhesion receptor. Up to three types of hemicellulases, an endoxylanase (XynII), an arabinofuranosidase (AbfB), and a β-xylosidase (XlnD), each bearing a C-terminal dockerin, were assembled onto the miniscaffoldin by high-affinity cohesin-dockerin interactions. Compared to uni- and bifunctional minihemicellulosomes, the resulting quaternary trifunctional complexes exhibited an enhanced rate of hydrolysis of arabinoxylan. Furthermore, with an integrated d-xylose-utilizing pathway, the recombinant yeast displaying the bifunctional minihemicellulosome CipA3-XynII-XlnD could simultaneously hydrolyze and ferment birchwood xylan to ethanol with a yield of 0.31 g per g of sugar consumed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.