ArticleApplied and environmental microbiology2012
Comparative analysis of eukaryotic marine microbial assemblages from 18S rRNA gene and gene transcript clone libraries by using different methods of extraction.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mission Tara Microplastics: a holistic set of protocols and data resources for the field investigation of plastic pollution along the land-sea continuum in Europe.Environmental science and pollution research international · 2025Article
- DNA extraction bias is more pronounced for microbial eukaryotes than for prokaryotes.MicrobiologyOpen · 2022Article
- Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China.Parasite (Paris, France) · 2022Article
- Role of Phylogenetic Structure in the Dynamics of Coastal Viral Assemblages.Applied and environmental microbiology · 2021Article
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- Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes.Applied and environmental microbiology · 2019Article
- Protist Diversity and Seasonal Dynamics in Skagerrak Plankton Communities as Revealed by Metabarcoding and Microscopy.The Journal of eukaryotic microbiology · 2019Article
- Induction and genetic identification of a callus-like growth developed in the brown algaEngineering in life sciences · 2019Article
- Single cell genomics yields a wide diversity of small planktonic protists across major ocean ecosystems.Scientific reports · 2019Article
- Eukaryotic microbes, principally fungi and labyrinthulomycetes, dominate biomass on bathypelagic marine snow.The ISME journal · 2017Article
- Sample Preservation, DNA or RNA Extraction and Data Analysis for High-Throughput Phytoplankton Community Sequencing.Frontiers in microbiology · 2017Article
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- Article
- Characterization of eukaryotic microbial diversity in hypersaline Lake Tyrrell, Australia.Frontiers in microbiology · 2013Article
- Reticulamoeba is a long-branched Granofilosean (Cercozoa) that is missing from sequence databases.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Eukaryotic marine microbes play pivotal roles in biogeochemical nutrient cycling and ecosystem function, but studies that focus on the protistan biogeography and genetic diversity lag-behind studies of other microbes. 18S rRNA PCR amplification and clone library sequencing are commonly used to assess diversity that is culture independent. However, molecular methods are not without potential biases and artifacts. In this study, we compare the community composition of clone libraries generated from the same water sample collected at the San Pedro Ocean Time Series (SPOTs) station in the northwest Pacific Ocean. Community composition was assessed using different cell lysis methods (chemical and mechanical) and the extraction of different nucleic acids (DNA and RNA reverse transcribed to cDNA) to build Sanger ABI clone libraries. We describe specific biases for ecologically important phylogenetic groups resulting from differences in nucleic acid extraction methods that will inform future designs of eukaryotic diversity studies, regardless of the target sequencing platform planned.
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