ArticleNucleic acids research2012
Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Transcriptional gene network involved in drought stress response: application for crop breeding in the context of climate change.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- RhMED15a-like, a subunit of the Mediator complex, is involved in the drought stress response in Rosa hybrida.BMC plant biology · 2024Article
- Molecular switching in transcription through splicing and proline-isomerization regulates stress responses in plants.Nature communications · 2024Article
- Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications.International journal of molecular sciences · 2020Review
- Specific functions for Mediator complex subunits from different modules in the transcriptional response of Arabidopsis thaliana to abiotic stress.Scientific reports · 2020Article
- Structural Basis for the Interaction between p53 Transactivation Domain and the Mediator Subunit MED25.Molecules (Basel, Switzerland) · 2018Article
- Structural disorder in plant proteins: where plasticity meets sessility.Cellular and molecular life sciences : CMLS · 2017Review
- Functional metabolomics as a tool to analyze Mediator function and structure in plants.PloS one · 2017Article
- The Multitalented MEDIATOR25.Frontiers in plant science · 2017Review
- Molecular mechanism of the priming by jasmonic acid of specific dehydration stress response genes in Arabidopsis.Epigenetics & chromatin · 2016Article
- Characterization of ERM transactivation domain binding to the ACID/PTOV domain of the Mediator subunit MED25.Nucleic acids research · 2015Article
- Article
- Structure, function and networks of transcription factors involved in abiotic stress responses.International journal of molecular sciences · 2013Article
- Stabilization of Arabidopsis DREB2A is required but not sufficient for the induction of target genes under conditions of stress.PloS one · 2013Article
- Conserved versatile master regulators in signalling pathways in response to stress in plants.AoB PLANTS · 2013Review
- MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis.Plant physiology · 2012Article
- Arabidopsis growth-regulating factor7 functions as a transcriptional repressor of abscisic acid- and osmotic stress-responsive genes, including DREB2A.The Plant cell · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator is a multiprotein coregulatory complex that conveys signals from DNA-bound transcriptional regulators to the RNA polymerase II transcription machinery in eukaryotes. The molecular mechanisms for how these signals are transmitted are still elusive. By using purified transcription factor Dreb2a, mediator subunit Med25 from Arabidopsis thaliana, and a combination of biochemical and biophysical methods, we show that binding of Dreb2a to its canonical DNA sequence leads to an increase in secondary structure of the transcription factor. Similarly, interaction between the Dreb2a and Med25 in the absence of DNA results in conformational changes. However, the presence of the canonical Dreb2a DNA-binding site reduces the affinity between Dreb2a and Med25. We conclude that transcription regulation is facilitated by small but distinct changes in energetic and structural parameters of the involved proteins.
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