ArticleMicrobes and environments2012
Diversity of cultivable methane-oxidizing bacteria in microsites of a rice paddy field: investigation by cultivation method and fluorescence in situ hybridization (FISH).
Article in Microbes and environments, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Expanding the Eco-collection of Methane-oxidizing Bacteria Inhabiting Rice Roots: Cultivation, Isolation, and Genomic Characterization of Isolates.Microbes and environments · 2025Article
- Methanotrophic Communities and Cultivation of Methanotrophs from Rice Paddy Fields Fertilized with Pig-livestock Biogas Digestive Effluent and Synthetic Fertilizer in the Vietnamese Mekong Delta.Microbes and environments · 2024Article
- Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies.Biology · 2023Article
- Type-specific quantification of particulate methane monooxygenase gene of methane-oxidizing bacteria at the oxic-anoxic interface of a surface paddy soil by digital PCR.Environmental microbiology reports · 2023Article
- Prospecting the significance of methane-utilizing bacteria in agriculture.World journal of microbiology & biotechnology · 2022Review
- Methane utilizing plant growth-promoting microbial diversity analysis of flooded paddy ecosystem of India.World journal of microbiology & biotechnology · 2021Article
- Cultivation of Important Methanotrophs From Indian Rice Fields.Frontiers in microbiology · 2021Article
- EDTA-FISH: A Simple and Effective Approach to Reduce Non-specific Adsorption of Probes in Fluorescence in situ Hybridization (FISH) for Environmental Samples.Microbes and environments · 2020Article
- Cultivated methanotrophs associated with rhizospheres of traditional rice landraces from Western India belong to3 Biotech · 2018Article
- Description of a methanotrophic strain BOH1, isolated from Al-Bohyriya well, Al-Ahsa City, Saudi Arabia.Saudi journal of biological sciences · 2017Article
- Topic of Influence, Methane and Microbes.Microbes and environments · 2017Article
- Deciphering Community Structure of Methanotrophs Dwelling in Rice Rhizospheres of an Indian Rice Field Using Cultivation and Cultivation-Independent Approaches.Microbial ecology · 2016Article
- Genome Characteristics of a Novel Type I Methanotroph (Sn10-6) Isolated from a Flooded Indian Rice Field.Microbial ecology · 2016Article
- Acid-Tolerant Moderately Thermophilic Methanotrophs of the Class Gammaproteobacteria Isolated From Tropical Topsoil with Methane Seeps.Frontiers in microbiology · 2016Article
- Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats.Microorganisms · 2015Article
- Article
- Low nitrogen fertilization adapts rice root microbiome to low nutrient environment by changing biogeochemical functions.Microbes and environments · 2014Article
- Cultivating yet-to-be cultivated microbes: the challenge continues.Microbes and environments · 2013Article
- Identification and phylogenetic characterization of cobalamin biosynthetic genes of Ensifer adhaerens.Microbes and environments · 2013Article
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5 authors.
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Abstract
The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), four type I, and nine type II MOB isolates were obtained from the highest dilution series of enrichment cultures. The type I MOB isolates included a novel species in the genus Methylomonas from floodwater and this is the first type I MOB strain isolated from floodwater of a rice paddy field. In the type I MOB, two isolates from stumps were closely related to Methylomonas spp.; one isolate obtained from rhizosphere soil was most related to Methyloccocus-Methylocaldum-Methylogaea clade. Almost all the type II MOB isolates were related to Methylocystis methanotrophs. FISH confirmed the presence of both types I and II MOB in all the microsites and in the related enrichment cultures. The study reported, for the first time, the diversity of cultivable methanotrophs including a novel species of type I MOB in rice paddy field compartments. Refining growth media and culture conditions, in combination with molecular approaches, will allow us to broaden our knowledge on the MOB community in the rice paddy field ecosystem and consequently to implement strategies for mitigating CH₄ emission from this ecosystem.
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