ArticleMolecular cell2012
Genome-wide functional annotation of dual-specificity protein- and lipid-binding modules that regulate protein interactions.
Article in Molecular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 62 citations in OpenAlex.
- PI(3,5)PScience advances · 2026Article
- Lipid Binding of SH2 Domains.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transmembrane Membrane Readers form a Novel Class of Proteins That Include Peripheral Phosphoinositide Recognition Domains and Viral Spikes.Membranes · 2022Review
- PI(4,5)PScience signaling · 2022Article
- Development of a novel spatiotemporal depletion system for cellular cholesterol.Journal of lipid research · 2022Article
- Review
- Review
- Attracted to membranes: lipid-binding domains in plants.Plant physiology · 2021Article
- Article
- Overexpression of rhophilin 2 promotes pancreatic ductal adenocarcinoma.Oncology letters · 2021Article
- Specificity in PDZ-peptide interaction networks: Computational analysis and review.Journal of structural biology: X · 2020Review
- PDZ Domains as Drug Targets.Advanced therapeutics · 2019Review
- CapZ integrates several signaling pathways in response to mechanical stiffness.The Journal of general physiology · 2019Article
- Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.Advances in experimental medicine and biology · 2019Review
- Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.International review of cell and molecular biology · 2019Review
- Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3,4,5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain.Cellular signalling · 2018Article
- Lipids at membrane contact sites: cell signaling and ion transport.EMBO reports · 2017Review
- Carboxy-terminal deletion of the HDL receptor reduces receptor levels in liver and steroidogenic tissues, induces hypercholesterolemia, and causes fatal heart disease.American journal of physiology. Heart and circulatory physiology · 2016Article
- Lipids Regulate Lck Protein Activity through Their Interactions with the Lck Src Homology 2 Domain.The Journal of biological chemistry · 2016Article
- Frizzled 7 and PIP2 binding by syntenin PDZ2 domain supports Frizzled 7 trafficking and signalling.Nature communications · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Emerging evidence indicates that membrane lipids regulate protein networking by directly interacting with protein-interaction domains (PIDs). As a pilot study to identify and functionally annodate lipid-binding PIDs on a genomic scale, we performed experimental and computational studies of PDZ domains. Characterization of 70 PDZ domains showed that ~40% had submicromolar membrane affinity. Using a computational model built from these data, we predicted the membrane-binding properties of 2,000 PDZ domains from 20 species. The accuracy of the prediction was experimentally validated for 26 PDZ domains. We also subdivided lipid-binding PDZ domains into three classes based on the interplay between membrane- and protein-binding sites. For different classes of PDZ domains, lipid binding regulates their protein interactions by different mechanisms. Functional studies of a PDZ domain protein, rhophilin 2, suggest that all classes of lipid-binding PDZ domains serve as genuine dual-specificity modules regulating protein interactions at the membrane under physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.