ArticleChemistry & biology2012
Targeted chemical-genetic regulation of protein stability in vivo.
Article in Chemistry & biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier.PLoS pathogens · 2016Article
- The Activity of Menkes Disease Protein ATP7A Is Essential for Redox Balance in Mitochondria.The Journal of biological chemistry · 2016Article
- Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.Molecular and cellular biology · 2016Article
- Adipose fatty acid oxidation is required for thermogenesis and potentiates oxidative stress-induced inflammation.Cell reports · 2015Article
- Engineering FKBP-Based Destabilizing Domains to Build Sophisticated Protein Regulation Systems.PloS one · 2015Article
- Article
- Article
- Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle.BMC biochemistry · 2014Article
- A genetically encoded metabolite sensor for malonyl-CoA.Chemistry & biology · 2012Article
- We see the light: chemical-genetic protein regulation.Chemistry & biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Loss- and gain-of-function transgenic models are powerful tools for understanding gene function in vivo but are limited in their ability to determine relative protein requirements. To determine cell-specific, temporal, or dose requirements of complex pathways, new methodology is needed. This is particularly important for deconstructing metabolic pathways that are highly interdependent and cross-regulated. We have combined mouse conditional transgenics and synthetic posttranslational protein stabilization to produce a broadly applicable strategy to regulate protein and pathway function in a cell-autonomous manner in vivo. Here, we show how a targeted chemical-genetic strategy can be used to alter fatty acid metabolism in a reombination and small-molecule-dependent manner in live behaving transgenic mice. This provides a practical, specific, and reversible means of manipulating metabolic pathways in adult mice to provide biological insight.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.