ArticleBMC genomics2012
The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 82 citations in OpenAlex.
- Integrated multi-dimensional modeling of non-model bacteria identifies engineering targets for acarbose biosynthesis optimization.Cell reports methods · 2026Article
- Evaluating the toxicity and efficacy of the endophytic bacteriumBiochemistry and biophysics reports · 2025Article
- Complete mitochondrial genomes of the Prototheca genus: comparative genomics and evolutionary insights.BMC genomics · 2025Article
- Targeting Transcriptional Regulators Affecting Acarbose Biosynthesis inMicroorganisms · 2024Article
- Discovery of thiazostatin D/E using UPLC-HR-MS2-based metabolomics and σ-factor engineering ofFrontiers in bioengineering and biotechnology · 2024Article
- The 4-α-Glucanotransferase AcbQ Is Involved in Acarbose Modification inMicroorganisms · 2023Article
- Acarbose May Function as a Competitive Exclusion Agent for the Producing Bacteria.ACS chemical biology · 2023Article
- Enhancement of acarbose production by genetic engineering and fed-batch fermentation strategy in Actinoplanes sp. SIPI12-34.Microbial cell factories · 2022Article
- Biochemical Characterization of GacI, a Bifunctional Glycosyltransferase-Phosphatase Enzyme Involved in Acarbose Biosynthesis inBiochemistry · 2022Article
- Effect of toyF on wuyiencin and toyocamycin production by Streptomyces albulus CK-15.World journal of microbiology & biotechnology · 2022Article
- Effects of Oral Glucose-Lowering Agents on Gut Microbiota and Microbial Metabolites.Frontiers in endocrinology · 2022Review
- Metabolites extracted from microorganisms as potential inhibitors of glycosidases (α-glucosidase and α-amylase): A review.Frontiers in microbiology · 2022Review
- Integrated Genomic and Functional Characterization of the Anti-diabetic Potential of Arthrobacter sp. SW1.Current microbiology · 2021Article
- Microbial Oligosaccharides with Biomedical Applications.Marine drugs · 2021Review
- The expression of the acarbose biosynthesis gene cluster in Actinoplanes sp. SE50/110 is dependent on the growth phase.BMC genomics · 2020Article
- A maltose-regulated large genomic region is activated by the transcriptional regulator MalT in Actinoplanes sp. SE50/110.Applied microbiology and biotechnology · 2020Article
- Absence of the highly expressed small carbohydrate-binding protein Cgt improves the acarbose formation in Actinoplanes sp. SE50/110.Applied microbiology and biotechnology · 2020Article
- Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110.Microbial cell factories · 2019Article
- Comparative functional genomics of the acarbose producers reveals potential targets for metabolic engineering.Synthetic and systems biotechnology · 2019Article
- Coexistence of Anticoagulant and Anti-vascular Calcification Activities inIranian journal of pharmaceutical research : IJPR · 2019Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundActinoplanes sp. SE50/110 is known as the wild type producer of the alpha-glucosidase inhibitor acarbose, a potent drug used worldwide in the treatment of type-2 diabetes mellitus. As the incidence of diabetes is rapidly rising worldwide, an ever increasing demand for diabetes drugs, such as acarbose, needs to be anticipated. Consequently, derived Actinoplanes strains with increased acarbose yields are being used in large scale industrial batch fermentation since 1990 and were continuously optimized by conventional mutagenesis and screening experiments. This strategy reached its limits and is generally superseded by modern genetic engineering approaches. As a prerequisite for targeted genetic modifications, the complete genome sequence of the organism has to be known.
resultsHere, we present the complete genome sequence of Actinoplanes sp. SE50/110 [GenBank:CP003170], the first publicly available genome of the genus Actinoplanes, comprising various producers of pharmaceutically and economically important secondary metabolites. The genome features a high mean G + C content of 71.32% and consists of one circular chromosome with a size of 9,239,851 bp hosting 8,270 predicted protein coding sequences. Phylogenetic analysis of the core genome revealed a rather distant relation to other sequenced species of the family Micromonosporaceae whereas Actinoplanes utahensis was found to be the closest species based on 16S rRNA gene sequence comparison. Besides the already published acarbose biosynthetic gene cluster sequence, several new non-ribosomal peptide synthetase-, polyketide synthase- and hybrid-clusters were identified on the Actinoplanes genome. Another key feature of the genome represents the discovery of a functional actinomycete integrative and conjugative element.
conclusionsThe complete genome sequence of Actinoplanes sp. SE50/110 marks an important step towards the rational genetic optimization of the acarbose production. In this regard, the identified actinomycete integrative and conjugative element could play a central role by providing the basis for the development of a genetic transformation system for Actinoplanes sp. SE50/110 and other Actinoplanes spp. Furthermore, the identified non-ribosomal peptide synthetase- and polyketide synthase-clusters potentially encode new antibiotics and/or other bioactive compounds, which might be of pharmacologic interest.
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