ArticlePloS one2012
Transcriptome profiling of testis during sexual maturation stages in Eriocheir sinensis using Illumina sequencing.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 94 citations in OpenAlex.
- Complex Sex Determination in the Grey MulletAnimals : an open access journal from MDPI · 2025Article
- Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn,Frontiers in genetics · 2022Article
- The Ovarian Development Genes of Bisexual and ParthenogeneticFrontiers in veterinary science · 2021Article
- Multiple transcriptome mining coupled with tissue specific molecular cloning and mass spectrometry provide insights into agatoxin-like peptide conservation in decapod crustaceans.General and comparative endocrinology · 2020Article
- Transcriptome analysis of hepatopancreas of Eriocheir sinensis with hepatopancreatic necrosis disease (HPND).PloS one · 2020Article
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- Transcriptomic Analysis Reveals Insights on Male Infertility inFrontiers in physiology · 2018Article
- Assembling large genomes: analysis of the stick insect (Clitarchus hookeri) genome reveals a high repeat content and sex-biased genes associated with reproduction.BMC genomics · 2017Article
- Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus.BMC genomics · 2017Article
- Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.PloS one · 2017Article
- Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland inBioMed research international · 2017Article
- Transcriptional responses in the hepatopancreas of Eriocheir sinensis exposed to deltamethrin.PloS one · 2017Article
- Transcriptome using Illumina sequencing reveals the traits of spermatogenesis and developing testes in Eriocheir sinensis.PloS one · 2017Article
- Exploring developmental gene toolkit and associated pathways in a potential new model crustacean using transcriptomic analysis.Development genes and evolution · 2016Article
- Article
- FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis.Bioscience reports · 2015Article
- Identification and Characterization of Reference Genes for Normalizing Expression Data from Red Swamp Crawfish Procambarus clarkii.International journal of molecular sciences · 2015Article
- ERK is involved in the process of acrosome reaction in vitro of the Chinese mitten crab, Eriocheir sinensis.Marine biotechnology (New York, N.Y.) · 2015Article
- A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).PloS one · 2015Article
Corrections and comments
- Erratum issuedJin, Xinkun [corrected to Jin, Xingkun].
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The testis is a highly specialized tissue that plays dual roles in ensuring fertility by producing spermatozoa and hormones. Spermatogenesis is a complex process, resulting in the production of mature sperm from primordial germ cells. Significant structural and biochemical changes take place in the seminiferous epithelium of the adult testis during spermatogenesis. The gene expression pattern of testis in Chinese mitten crab (Eriocheir sinensis) has not been extensively studied, and limited genetic research has been performed on this species. The advent of high-throughput sequencing technologies enables the generation of genomic resources within a short period of time and at minimal cost. In the present study, we performed de novo transcriptome sequencing to produce a comprehensive transcript dataset for testis of E. sinensis. In two runs, we produced 25,698,778 sequencing reads corresponding with 2.31 Gb total nucleotides. These reads were assembled into 342,753 contigs or 141,861 scaffold sequences, which identified 96,311 unigenes. Based on similarity searches with known proteins, 39,995 unigenes were annotated based on having a Blast hit in the non-redundant database or ESTscan results with a cut-off E-value above 10(-5). This is the first report of a mitten crab transcriptome using high-throughput sequencing technology, and all these testes transcripts can help us understand the molecular mechanisms involved in spermatogenesis and testis maturation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.