ArticlePloS one2012
Identifying new therapeutic targets via modulation of protein corona formation by engineered nanoparticles.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Article
- Nanotechnology Meets Oncology: A Perspective on the Role of the Personalized Nanoparticle-Protein Corona in the Development of Technologies for Pancreatic Cancer Detection.International journal of molecular sciences · 2022Review
- Camels' biological fluids contained nanobodies: promising avenue in cancer therapy.Cancer cell international · 2022Review
- Gold Nanoparticles Contact with Cancer Cell: A Brief Update.International journal of molecular sciences · 2022Review
- Experimental conditions influence the formation and composition of the corona around gold nanoparticles.Cancer nanotechnology · 2021Article
- A Novel Nanoproteomic Approach for the Identification of Molecular Targets Associated with Thyroid Tumors.Nanomaterials (Basel, Switzerland) · 2020Article
- Ubiquitin-binding associated protein 2 regulates KRAS activation and macropinocytosis in pancreatic cancer.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Insights into Characterization Methods and Biomedical Applications of Nanoparticle-Protein Corona.Materials (Basel, Switzerland) · 2020Review
- Analysing the nanoparticle-protein corona for potential molecular target identification.Journal of controlled release : official journal of the Controlled Release Society · 2020Review
- Unveiling the pitfalls of the protein corona of polymeric drug nanocarriers.Drug delivery and translational research · 2020Review
- Isolation methods for particle protein corona complexes from protein-rich matrices.Nanoscale advances · 2020Review
- Nanoparticle Interactions with the Tumor Microenvironment.Bioconjugate chemistry · 2019Review
- A health concern regarding the protein corona, aggregation and disaggregation.Biochimica et biophysica acta. General subjects · 2019Review
- Mannose-decorated hybrid nanoparticles for enhanced macrophage targeting.Biochemistry and biophysics reports · 2019Article
- Probing Cellular Processes Using Engineered Nanoparticles.Bioconjugate chemistry · 2018Review
- Nanoparticle-Assisted Metabolomics.Metabolites · 2018Review
- Cellular processing of gold nanoparticles: CE-ICP-MS evidence for the speciation changes in human cytosol.Analytical and bioanalytical chemistry · 2018Article
- Short-chained oligo(ethylene oxide)-functionalized gold nanoparticles: realization of significant protein resistance.Analytical and bioanalytical chemistry · 2018Article
- Current Challenges toward In Vitro Cellular Validation of Inorganic Nanoparticles.Bioconjugate chemistry · 2017Review
- Protein corona: a new approach for nanomedicine design.International journal of nanomedicine · 2017Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundWe introduce a promising methodology to identify new therapeutic targets in cancer. Proteins bind to nanoparticles to form a protein corona. We modulate this corona by using surface-engineered nanoparticles, and identify protein composition to provide insight into disease development. METHODS/PRINCIPAL
findingsUsing a family of structurally homologous nanoparticles we have investigated the changes in the protein corona around surface-functionalized gold nanoparticles (AuNPs) from normal and malignant ovarian cell lysates. Proteomics analysis using mass spectrometry identified hepatoma-derived growth factor (HDGF) that is found exclusively on positively charged AuNPs ((+)AuNPs) after incubation with the lysates. We confirmed expression of HDGF in various ovarian cancer cells and validated binding selectivity to (+)AuNPs by Western blot analysis. Silencing of HDGF by siRNA resulted s inhibition in proliferation of ovarian cancer cells.
conclusionWe investigated the modulation of protein corona around surface-functionalized gold nanoparticles as a promising approach to identify new therapeutic targets. The potential of our method for identifying therapeutic targets was demonstrated through silencing of HDGF by siRNA, which inhibited proliferation of ovarian cancer cells. This integrated proteomics, bioinformatics, and nanotechnology strategy demonstrates that protein corona identification can be used to discover novel therapeutic targets in cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.