ArticlePloS one2012
Cigarette smoke affects keratinocytes SRB1 expression and localization via H2O2 production and HNE protein adducts formation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 2 syntheses or guidelines pooled it, 106 citations in OpenAlex.
- Environmental exposures and atopic dermatitis: an umbrella review of systematic reviews and meta-analyses.Frontiers in public health · 2026Pooled it
- Association of atopic dermatitis with smoking: A systematic review and meta-analysis.Journal of the American Academy of Dermatology · 2016Pooled it
- Exposome involvement in the development of acne vulgaris.Frontiers in immunology · 2026Review
- Ashes to Rashes: An Exploration of the Intersection Between Smoking and Cutaneous Lupus Erythematosus.Biomolecules · 2025Review
- Keratinocyte SR-B1 expression and targeting in cytokine-driven skin inflammation.Communications medicine · 2025Article
- Oxidative stress products and managements in atopic dermatitis.Frontiers in medicine · 2025Review
- Lifestyle Factors in the Clinical Manifestation and Management of Atopic Dermatitis.Archives of internal medicine research · 2025Article
- Topical Application of M89PF Containing Vichy Mineralising Water and Probiotic Fractions Prevents Cutaneous Damage Induced by Exposure to UV and OClinical, cosmetic and investigational dermatology · 2023Article
- Antioxidant, Anti-Inflammation and Antiaging Activities ofAntioxidants (Basel, Switzerland) · 2022Article
- Ultraviolet Light Protection: Is It Really Enough?Antioxidants (Basel, Switzerland) · 2022Review
- Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.Respiratory research · 2022Article
- Inflammasome involvement in CS-induced damage in HaCaT keratinocytes.In vitro cellular & developmental biology. Animal · 2022Article
- Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle.Histochemistry and cell biology · 2021Article
- A comprehensive topical antioxidant inhibits oxidative stress induced by blue light exposure and cigarette smoke in human skin tissue.Journal of cosmetic dermatology · 2021Article
- A Comprehensive Review on Source, Types, Effects, Nanotechnology, Detection, and Therapeutic Management of Reactive Carbonyl Species Associated with Various Chronic Diseases.Antioxidants (Basel, Switzerland) · 2020Review
- Article
- The exposome in atopic dermatitis.Allergy · 2020Review
- The Skin Epilipidome in Stress, Aging, and Inflammation.Frontiers in endocrinology · 2020Review
- Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter.Biomolecules & therapeutics · 2019Article
- Scavenger Receptor Class B type 1 (SR-B1) and the modifiable risk factors of stroke.Chinese neurosurgical journal · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scavenger Receptor B1 (SR-B1), also known as HDL receptor, is involved in cellular cholesterol uptake. Stratum corneum (SC), the outermost layer of the skin, is composed of more than 25% cholesterol. Several reports support the view that alteration of SC lipid composition may be the cause of impaired barrier function which gives rise to several skin diseases. For this reason the regulation of the genes involved in cholesterol uptake is of extreme significance for skin health. Being the first shield against external insults, the skin is exposed to several noxious substances and among these is cigarette smoke (CS), which has been recently associated with various skin pathologies. In this study we first have shown the presence of SR-B1 in murine and human skin tissue and then by using immunoblotting, immunoprecipitation, RT-PCR, and confocal microscopy we have demonstrated the translocation and the subsequent lost of SR-B1 in human keratinocytes (cell culture model) after CS exposure is driven by hydrogen peroxide (H(2)O(2)) that derives not only from the CS gas phase but mainly from the activation of cellular NADPH oxidase (NOX). This effect was reversed when the cells were pretreated with NOX inhibitors or catalase. Furthermore, CS caused the formation of SR-B1-aldheydes adducts (acrolein and 4-hydroxy-2-nonenal) and the increase of its ubiquitination, which could be one of the causes of SR-B1 loss. In conclusion, exposure to CS, through the production of H(2)O(2), induced post-translational modifications of SR-B1 with the consequence lost of the receptor and this may contribute to the skin physiology alteration as a consequence of the variation of cholesterol uptake.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.