ArticleJournal of clinical microbiology2012
Novel molecular method for identification of Streptococcus pneumoniae applicable to clinical microbiology and 16S rRNA sequence-based microbiome studies.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Surveillance of invasive pneumococcal disease in Germany: surge in PCV13 serotypes and new challenges, 2017 to 2024.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026Article
- Re-evaluation of boundaries ofInternational journal of systematic and evolutionary microbiology · 2025Article
- Method development and clinical validation of LAMP-CRISPR/Cas12a for rapid detection of respiratory pathogens in children.Frontiers in pediatrics · 2025Article
- Development of an automated amplicon-based next-generation sequencing pipeline for rapid detection of bacteria and fungi directly from clinical specimens.Journal of clinical microbiology · 2024Article
- Genomic Features and Comparative Genomic Analysis of Streptococcus sp. v1. nov., Isolated from an Endophthalmitis Patient.Current microbiology · 2023Article
- Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results.Journal of clinical laboratory analysis · 2022Article
- Streptococcus pseudopneumoniae: Use of Whole-Genome Sequences To Validate Species Identification Methods.Journal of clinical microbiology · 2021Article
- Development of Loop-Mediated Isothermal Amplification Assay TargetingFrontiers in microbiology · 2021Article
- Identification of Streptococcus pneumoniae and other Mitis streptococci: importance of molecular methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Relationships among streptococci from the mitis group, misidentified as Streptococcus pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Profiling of Oral Bacterial Communities.Journal of dental research · 2020Review
- Serotype Distribution and Antimicrobial Susceptibility ofFrontiers in microbiology · 2020Article
- Phosphatidylcholine Biosynthesis in Mitis Group Streptococci via Host Metabolite Scavenging.Journal of bacteriology · 2019Article
- Pneumococcal carriage among children under five in Accra, Ghana, five years after the introduction of pneumococcal conjugate vaccine.BMC pediatrics · 2019Article
- Streptococcus gwangjuense sp. nov., Isolated from Human Pericoronitis.Current microbiology · 2019Article
- Molecular characterization and epidemiology of Streptococcus pneumoniae serotype 24F in Denmark.Scientific reports · 2019Article
- Identification of Streptococcus pneumoniae by a real-time PCR assay targeting SP2020.Scientific reports · 2019Article
- iDS372, a Phenotypically Reconciled Model for the Metabolism ofFrontiers in microbiology · 2019Article
- Airway microbiome research: a modern perspective on surveillance cultures?Annals of translational medicine · 2017Review
- Sequencing of the variable region ofOpen biology · 2017Article
Corrections and comments
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The close phylogenetic relationship of the important pathogen Streptococcus pneumoniae and several species of commensal streptococci, particularly Streptococcus mitis and Streptococcus pseudopneumoniae, and the recently demonstrated sharing of genes and phenotypic traits previously considered specific for S. pneumoniae hamper the exact identification of S. pneumoniae. Based on sequence analysis of 16S rRNA genes of a collection of 634 streptococcal strains, identified by multilocus sequence analysis, we detected a cytosine at position 203 present in all 440 strains of S. pneumoniae but replaced by an adenosine residue in all strains representing other species of mitis group streptococci. The S. pneumoniae-specific sequence signature could be demonstrated by sequence analysis or indirectly by restriction endonuclease digestion of a PCR amplicon covering the site. The S. pneumoniae-specific signature offers an inexpensive means for validation of the identity of clinical isolates and should be used as an integrated marker in the annotation procedure employed in 16S rRNA-based molecular studies of complex human microbiotas. This may avoid frequent misidentifications such as those we demonstrate to have occurred in previous reports and in reference sequence databases.
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