ArticleJournal of controlled release : official journal of the Controlled Release Society2012
Ligands located within a cholesterol domain enhance gene delivery to the target tissue.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- The Effect of Repeat Administration of Lipoplexes on Gene Delivery, Biodistribution, and Cytokine Response in Immunocompetent Tumor-Bearing Mice.Journal of pharmaceutical sciences · 2022Article
- Physicochemical Factors That Influence the Biocompatibility of Cationic Liposomes and Their Ability to Deliver DNA to the Nuclei of Ovarian Cancer SK-OV-3 Cells.Materials (Basel, Switzerland) · 2021Article
- The Use of Lactose as an Alternative Coating for Nanoparticles.Journal of pharmaceutical sciences · 2020Article
- A novel method for conjugating the terminal amine of peptide ligands to cholesterol: synthesis iRGD-cholesterol.Therapeutic delivery · 2019Article
- Nanoparticle uptake by circulating leukocytes: A major barrier to tumor delivery.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Folate-mediated chemotherapy and diagnostics: An updated review and outlook.Journal of controlled release : official journal of the Controlled Release Society · 2017Review
- Assessing the effect of a nude mouse model on nanoparticle-mediated gene delivery.Drug delivery and translational research · 2017Article
- Relating toxicity to transfection: using sphingosine to maintain prolonged expression in vitro.Molecular pharmaceutics · 2015Article
- α, ω-Cholesterol-functionalized low molecular weight polyethylene glycol as a novel modifier of cationic liposomes for gene delivery.International journal of molecular sciences · 2014Article
- Examination of the specificity of tumor cell derived exosomes with tumor cells in vitro.Biochimica et biophysica acta · 2014Article
- Self-assembled micelles of novel amphiphilic copolymer cholesterol-coupled F68 containing cabazitaxel as a drug delivery system.International journal of nanomedicine · 2014Article
- The effects of lipoplex formulation variables on the protein corona and comparisons with in vitro transfection efficiency.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Cholesterol domains enhance transfection.Therapeutic delivery · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Targeted gene delivery provides enormous potential for clinical treatment of many incurable diseases. Liposomes formulated with targeting ligands have been tested extensively both in vitro and in vivo, and many studies have strived to identify more efficacious ligands. However, the environment of the ligand within the delivery vehicle is generally not considered, and this study assesses the effect of ligand microenvironment by utilizing a lipoplex possessing a cholesterol domain. Our recent work has shown that the presence of the targeting ligand within the cholesterol domain promotes more productive transfection in cultured cells. In the present study, lipoplexes having the identical lipid composition were formulated with different conjugates of the folate ligand such that the ligand was included in, or excluded from, the cholesterol domain. The effect of locating the ligand within the cholesterol domain was then tested in a xenograft tumor model in mice. Lipoplexes that included the ligand within the cholesterol domain showed significantly higher luciferase expression and plasmid accumulation in tumors as compared to lipoplexes in which the ligand was excluded from the domain. These results demonstrate that the microenvironment of the ligand can affect gene delivery to tumors, and show that ligand-mediated delivery can be enhanced by locating targeting ligands within a cholesterol domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.