ArticlePloS one2012
Rapid susceptibility testing and microcolony analysis of Candida spp. cultured and imaged on porous aluminum oxide.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Multispectral Infrared Colony Phenotyping for High-Throughput Microbiological Control of Waters.Journal of biophotonics · 2026Article
- Octenidine effectively reduces Candida auris colonisation on human skin.Scientific reports · 2025Article
- Characterization and virulence of yeasts associated with neonatal thrush.BMC microbiology · 2025Article
- Microbial Identification Through Multispectral Infrared Imaging of Colonies: A New Type of Morpho-Spectral Fingerprinting.Microbial biotechnology · 2025Article
- Early phenotypic detection of fluconazole- and anidulafungin-resistant Candida glabrata isolates.The Journal of antimicrobial chemotherapy · 2022Article
- Antifungal Activity and Biocompatibility of α-AgVOFrontiers in bioengineering and biotechnology · 2022Article
- Non-antifungal drugs inhibit growth, morphogenesis and biofilm formation in Candida albicans.The Journal of antibiotics · 2021Article
- Rapid Antifungal Susceptibility Testing of Yeasts and Molds by MALDI-TOF MS: A Systematic Review and Meta-Analysis.Journal of fungi (Basel, Switzerland) · 2021Review
- Can We Improve Antifungal Susceptibility Testing?Frontiers in cellular and infection microbiology · 2021Review
- Antifungal Susceptibility Testing ofACS infectious diseases · 2020Article
- Mechanism of Candida pathogenesis: revisiting the vital drivers.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Antifungal Susceptibility Testing: Current Approaches.Clinical microbiology reviews · 2020Review
- Candida sp. Infections in Patients with Diabetes Mellitus.Journal of clinical medicine · 2019Review
- Relationship between Sap prevalence and biofilm formation among resistant clinical isolates ofAfrican health sciences · 2018Article
- Anti-Antimicrobial resistance and infection control · 2017Article
- Physically Triggered Morphology Changes in a NovelFrontiers in microbiology · 2017Article
- Antifungal activity of amphotericin B and voriconazole against the biofilms and biofilm-dispersed cells of Candida albicans employing a newly developed in vitro pharmacokinetic model.Annals of clinical microbiology and antimicrobials · 2015Article
- Rapid antifungal susceptibility testing by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis.Journal of clinical microbiology · 2013Article
- Rapid clinical bacteriology and its future impact.Annals of laboratory medicine · 2013Review
- Anti-candidal activity of genetically engineered histatin variants with multiple functional domains.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcquired resistance to antifungal agents now supports the introduction of susceptibility testing for species-drug combinations for which this was previously thought unnecessary. For pathogenic yeasts, conventional phenotypic testing needs at least 24 h. Culture on a porous aluminum oxide (PAO) support combined with microscopy offers a route to more rapid results.
methodsMicrocolonies of Candida species grown on PAO were stained with the fluorogenic dyes Fun-1 and Calcofluor White and then imaged by fluorescence microscopy. Images were captured by a charge-coupled device camera and processed by publicly available software. By this method, the growth of yeasts could be detected and quantified within 2 h. Microcolony imaging was then used to assess the susceptibility of the yeasts to amphotericin B, anidulafungin and caspofungin (3.5 h culture), and voriconazole and itraconazole (7 h culture). SIGNIFICANCE: Overall, the results showed good agreement with EUCAST (86.5% agreement; n = 170) and E-test (85.9% agreement; n = 170). The closest agreement to standard tests was found when testing susceptibility to amphotericin B and echinocandins (88.2 to 91.2%) and the least good for the triazoles (79.4 to 82.4%). Furthermore, large datasets on population variation could be rapidly obtained. An analysis of microcolonies revealed subtle effects of antimycotics on resistant strains and below the MIC of sensitive strains, particularly an increase in population heterogeneity and cell density-dependent effects of triazoles. Additionally, the method could be adapted to strain identification via germ tube extension. We suggest PAO culture is a rapid and versatile method that may be usefully adapted to clinical mycology and has research applications.
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