Evidence map›Paper›PMID 22438973›Full record

ArticlePloS one2012

Comparison of promoter hypermethylation pattern in salivary rinses collected with and without an exfoliating brush from patients with HNSCC.

Wenyue Sun, David Zaboli, Yan Liu, Demetri Arnaoutakis, Tanbir Khan, Hao Wang, Wayne Koch, Zubair Khan, Joseph A Califano

Abstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wenyue SunDepartment of Otolaryngology-Head and Neck Surgery, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins Medical Institutions, Baltimore, Maryland, United States of America.
David Zaboli
Yan Liu
Demetri Arnaoutakis
Tanbir Khan
Hao Wang
Wayne Koch
Zubair Khan
Joseph A Califano

Funding

INTEGRATED DEVELOPMENT OF NOVEL MOLECULAR MARKERSU01CA084986 · NCI · JOHNS HOPKINS UNIVERSITY · PI SIDRANSKY, DAVID · 1999 to 2014
$33.8M
TUMOR SUPPRESOR GENE PROMOTER HYPERMETHYLATION FOR DETECTION OF HEAD AND NECKP50DE019032 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI SIDRANSKY, DAVID · 2007 to 2016
$27.1M
RISK OF ORAL EPITHELIAL DYSPLASIA IN PUERTO RICOU54DE014257 · NIDCR · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MORSE, DOUGLAS E · 2001 to 2007
$7.3M
Integrative Pathway Analysis of Eqigenomic/transcriptional Alteration in HNSCCRC1DE020324 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI CALIFANO, JOSEPH A · 2009 to 2010
$918k
Validation of epigenetic biomarkers of head and neck cancer progression--OLDRC2DE020789 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI CALIFANO, JOSEPH A · 2009 to 2010
$546k
NCI NIH HHS U01 CA084986NCI NIH HHS U01-CA084986NIDCR NIH HHS P50 DE019032NIDCR NIH HHS P50DE019032NIDCR NIH HHS RC1 DE020324NIDCR NIH HHS RC1DE020324NIDCR NIH HHS RC2 DE020789NIDCR NIH HHS RC2DE020789NIDCR NIH HHS U54 DE014257NIDCR NIH HHS U54DE14257
6 · The paper itself

Abstract

backgroundSalivary rinses have been recently proposed as a valuable resource for the development of epigenetic biomarkers for detection and monitoring of head and neck squamous cell carcinoma (HNSCC). Both salivary rinses collected with and without an exfoliating brush from patients with HNSCC are used in detection of promoter hypermethylation, yet their correlation of promoter hypermethylation has not been evaluated. This study was to evaluate the concordance of promoter hypermethylation between salivary rinses collected with and without an exfoliating brush from patients with HNSCC. METHODOLGY: 57 paired salivary rinses collected with or without an exfoliating brush from identical HNSCC patients were evaluated for promoter hypermethylation status using Quantitative Methylation-Specific PCR. Target tumor suppressor gene promoter regions were selected based on our previous studies describing a panel for HNSCC screening and surveillance, including P16, CCNA1, DCC, TIMP3, MGMT, DAPK and MINT31. PRINCIPAL

findingsIn salivary rinses collected with and without brush, frequent methylation was detected in P16 (8.8% vs. 5.2%), CCNA1 (26.3% vs. 22.8%), DCC (33.3% vs. 29.8%), TIMP3 (31.6% vs. 36.8%), MGMT (29.8% vs. 38.6%), DAPK (14.0% vs. 19.2%), and MINT31 (10.5% vs. 8.8%). Spearman's rank correlation coefficient showed a positive correlation between salivary rinses collected with and without brush for P16 (ρ = 0.79), CCNA1 (ρ = 0.61), DCC (ρ = 0.58), TIMP3 (ρ = 0.10), MGMT (ρ = 0.70), DAPK (ρ = 0.51) and MINT31 (ρ = 0.72) (P<0.01). The percent agreement of promoter methylation between salivary rinses with brush and without brush were 96.5% for P16, 82.5% for CCNA1, 78.9% for DCC, 59.7% for TIMP3, 84.2% for MGMT, 84.2% for DAPK, and 94.7% for MINT31.

conclusionsOur study demonstrated strong correlations of gene promoter hypermethylation between salivary rinses collected with and without an exfoliating brush. Salivary rinse collection without using an exfoliating brush may offer a cost effective, rapid, non-invasive, and reliable means for development of epigenetic salivary rinse biomarkers.

Indexed as

DNA MethylationPromoter Regions, GeneticAdultAgedAged, 80 and overApoptosis Regulatory ProteinsCalcium-Calmodulin-Dependent Protein KinasesCarcinoma, Squamous CellCyclin A1Death-Associated Protein KinasesDNA Modification MethylasesDNA, NeoplasmDNA Repair EnzymesEpigenesis, GeneticFemaleGenes, DCCApoptosis Regulatory ProteinsCalcium-Calmodulin-Dependent Protein KinasesCCNA1 protein, humanCyclin A1Death-Associated Protein KinasesDNA Modification MethylasesDNA, NeoplasmDNA Repair EnzymesMGMT protein, humanTissue Inhibitor of Metalloproteinase-3Tumor Suppressor Proteins

Identifiers

PMID22438973
PMCPMC3306276

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.