ArticlePloS one2012
Development of multigene expression signature maps at the protein level from digitized immunohistochemistry slides.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Clinical Validation of Targeted Next Generation Sequencing for Colon and Lung Cancers.PloS one · 2015Trial
- Development and validation of a novel hypoxia-related signature for prognostic and immunogenic evaluation in head and neck squamous cell carcinoma.Frontiers in oncology · 2022Article
- ANHIR: Automatic Non-Rigid Histological Image Registration Challenge.IEEE transactions on medical imaging · 2020Article
- Signature maps for automatic identification of prostate cancer from colorimetric analysis of H&E- and IHC-stained histopathological specimens.Scientific reports · 2019Article
- Raman-Encoded Molecular Imaging with Topically Applied SERS Nanoparticles for Intraoperative Guidance of Lumpectomy.Cancer research · 2017Article
- Use of the QIAGEN GeneReader NGS system for detection of KRAS mutations, validated by the QIAGEN Therascreen PCR kit and alternative NGS platform.BMC cancer · 2017Article
- Alpha-enolase is a potential prognostic marker in clear cell renal cell carcinoma.Clinical & experimental metastasis · 2015Article
- Registration of whole immunohistochemical slide images: an efficient way to characterize biomarker colocalization.Journal of the American Medical Informatics Association : JAMIA · 2015Article
- Intratumor mapping of intracellular water lifetime: metabolic images of breast cancer?NMR in biomedicine · 2014Article
- Evaluation of protein biomarkers of prostate cancer aggressiveness.BMC cancer · 2014Article
- Expression of FAP, ADAM12, WISP1, and SOX11 is heterogeneous in aggressive fibromatosis and spatially relates to the histologic features of tumor activity.Cancer medicine · 2014Article
- Quantitative comparison of immunohistochemical staining measured by digital image analysis versus pathologist visual scoring.Diagnostic pathology · 2012Article
- Article
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Authors and funding
6 authors.
Funding
Abstract
Molecular classification of diseases based on multigene expression signatures is increasingly used for diagnosis, prognosis, and prediction of response to therapy. Immunohistochemistry (IHC) is an optimal method for validating expression signatures obtained using high-throughput genomics techniques since IHC allows a pathologist to examine gene expression at the protein level within the context of histologically interpretable tissue sections. Additionally, validated IHC assays may be readily implemented as clinical tests since IHC is performed on routinely processed clinical tissue samples. However, methods have not been available for automated n-gene expression profiling at the protein level using IHC data. We have developed methods to compute expression level maps (signature maps) of multiple genes from IHC data digitized on a commercial whole slide imaging system. Areas of cancer for these expression level maps are defined by a pathologist on adjacent, co-registered H&E slides, allowing assessment of IHC statistics and heterogeneity within the diseased tissue. This novel way of representing multiple IHC assays as signature maps will allow the development of n-gene expression profiling databases in three dimensions throughout virtual whole organ reconstructions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.