ArticlePloS one2012
The Cryptosporidium parvum transcriptome during in vitro development.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 107 citations in OpenAlex.
- Integrated Transcriptomic Profiling and In Vitro Evaluation of Garcinia Mangostana Phytochemicals Against Cryptosporidium Parvum.Cell biochemistry and biophysics · 2026Article
- Functional Characterization and Inhibition Analysis of a Glutathione Transferase fromPharmaceuticals (Basel, Switzerland) · 2026Article
- Host cell KRT-18 as a non-redox dependent interactor of Cryptosporidium parvum PDI enables parasite infection.Communications biology · 2025Article
- Gene regulation inCurrent research in parasitology & vector-borne diseases · 2025Article
- Exploring the impact of digestive physicochemical parameters of adults and infants on the pathophysiology of Cryptosporidium parvum using the dynamic TIM-1 gastrointestinal model.Gut pathogens · 2024Article
- The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence.Cell reports · 2024Article
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- Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 inInternational journal of molecular sciences · 2024Article
- A single-pass type I membrane protein, mannose-specific L-type lectin, potentially involved in the adhesion and invasion of Cryptosporidium parvum.Parasite (Paris, France) · 2024Article
- Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1).Parasitology research · 2023Article
- Combination of inhibitors for two glycolytic enzymes portrays high synergistic efficacy againstAntimicrobial agents and chemotherapy · 2023Article
- InvestigatingInternational journal of molecular sciences · 2023Review
- Mass Spectrometry Imaging of In VitroBiomolecules · 2023Article
- Article
- Characterization of CpCaM, a protein potentially involved in the growth of Cryptosporidium parvum.Parasitology research · 2023Article
- Enrichment and proteomic identification of Cryptosporidium parvum oocyst wall.Parasites & vectors · 2022Article
- An update onJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Characterization of Dense Granule Metalloproteinase INS-16 inInternational journal of molecular sciences · 2022Article
- Small and intermediate size structural RNAs in the unicellular parasiteMicrobial genomics · 2022Article
- Protein Kinase C-α Is a Gatekeeper ofInfection and immunity · 2022Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cryptosporidiosis is caused by an obligate intracellular parasite that has eluded global transcriptional or proteomic analysis of the intracellular developmental stages. The transcript abundance for 3,302 genes (87%) of the Cryptosporidium parvum protein coding genome was elucidated over a 72 hr infection within HCT8 cells using Real Time-PCR. The parasite had detectable transcription of all genes in vitro within at least one time point tested, and adjacent genes were not co-regulated. Five genes were not detected within the first 24 hr of infection, one containing two AP2 domains. The fewest genes detected were at 2 hr post infection, while 30% (985) of the genes have their highest expression at 48 and/or 72 hr. Nine expression clusters were formed over the entire 72 hr time course and indicate patterns of transcriptional increases at each of the 7 time points collected except 36 hr, including genes paralleling parasite 18S rRNA transcript levels. Clustering within only the first 24 hr of infection indicates spikes in expression at each of the 4 time points, a group paralleling 18S rRNA transcript levels, and a cluster with peaks at both 6 and 24 hr. All genes were classified into 18 functional categories, which were unequally distributed across clusters. Expression of metabolic, ribosomal and proteasome proteins did not parallel 18S rRNA levels indicating distinct biochemical profiles during developmental stage progression. Proteins involved in translation are over-represented at 6 hr, while structural proteins are over-represented at 12 hr. Standardization methods identified 107 genes with <80% at a single of its total expression at a single time point over 72 hr. This comprehensive transcriptome of the intracellular stages of C. parvum provides insight for understanding its complex development following parasitization of intestinal epithelial cells.
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