ArticleSensors (Basel, Switzerland)2012
A platform for combined DNA and protein microarrays based on total internal reflection fluorescence.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Design of Hydrogel Silk-Based Microarrays and Molecular Beacons for Reagentless Point-of-Care Diagnostics.Frontiers in bioengineering and biotechnology · 2022Article
- Phosphorylation of NMDA receptors by cyclin B/CDK1 modulates calcium dynamics and mitosis.Communications biology · 2020Article
- Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection.Scientific reports · 2019Article
- Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels modulates cell migration and wound healing.Communications biology · 2019Article
- A novel family of mammalian transmembrane proteins involved in cholesterol transport.Scientific reports · 2017Article
- A cholesterol-binding domain in STIM1 modulates STIM1-Orai1 physical and functional interactions.Scientific reports · 2016Article
- Evanescent wave fluorescence biosensors: Advances of the last decade.Biosensors & bioelectronics · 2016Review
- Shrink-induced silica multiscale structures for enhanced fluorescence from DNA microarrays.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Point-of-care diagnostic tools to detect circulating microRNAS as biomarkers of disease.Sensors (Basel, Switzerland) · 2014Review
- A cholesterol recognition amino acid consensus domain in GP64 fusion protein facilitates anchoring of baculovirus to mammalian cells.Journal of virology · 2013Article
- Microarray technology for major chemical contaminants analysis in food: current status and prospects.Sensors (Basel, Switzerland) · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel microarray technology based on total internal reflection fluorescence (TIRF) in combination with DNA and protein bioassays immobilized at the TIRF surface. Unlike conventional microarrays that exhibit reduced signal-to-background ratio, require several stages of incubation, rinsing and stringency control, and measure only end-point results, our TIRF microarray technology provides several orders of magnitude better signal-to-background ratio, performs analysis rapidly in one step, and measures the entire course of association and dissociation kinetics between target DNA and protein molecules and the bioassays. In many practical cases detection of only DNA or protein markers alone does not provide the necessary accuracy for diagnosing a disease or detecting a pathogen. Here we describe TIRF microarrays that detect DNA and protein markers simultaneously, which reduces the probabilities of false responses. Supersensitive and multiplexed TIRF DNA and protein microarray technology may provide a platform for accurate diagnosis or enhanced research studies. Our TIRF microarray system can be mounted on upright or inverted microscopes or interfaced directly with CCD cameras equipped with a single objective, facilitating the development of portable devices. As proof-of-concept we applied TIRF microarrays for detecting molecular markers from Bacillus anthracis, the pathogen responsible for anthrax.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.