Evidence map›Paper›PMID 22438725›Full record

ReviewSensors (Basel, Switzerland)2012

Pseudomonas fluorescens HK44: lessons learned from a model whole-cell bioreporter with a broad application history.

Josef Trögl, Archana Chauhan, Steven Ripp, Alice C Layton, Gabriela Kuncová, Gary S Sayler

Abstract readReview
In one paragraph

Review in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Review
  3. Advances in biosensors for bacterial detection and identification.World journal of microbiology & biotechnology · 2025
    Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Detection of organic compounds with whole-cell bioluminescent bioassays.Advances in biochemical engineering/biotechnology · 2014
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Josef TröglFaculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Králova Výšina 3132/7, 400 96 Ústí nad Labem, Czech Republic. josef.trogl@ujep.cz
Archana Chauhan
Steven Ripp
Alice C Layton
Gabriela Kuncová
Gary S Sayler

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution.

Indexed as

Biological AssayBiosensing TechniquesEnvironmental MonitoringEquipment DesignEquipment Failure AnalysisPseudomonas fluorescensSpectrometry, Fluorescencebioluminescencebioreporterbiosensorslux genesPseudomonas fluorescens HK44

Identifiers

PMID22438725
PMCPMC3304127

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.