ArticleSensors (Basel, Switzerland)2012
Miniaturized protein microarray with internal calibration as point-of-care device for diagnosis of neonatal sepsis.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Recent Progresses in Optical Biosensors for Interleukin 6 Detection.Biosensors · 2023Review
- NiCoP/g-CSensors (Basel, Switzerland) · 2023Article
- Article
- Designing electrochemical microfluidic multiplexed biosensors for on-site applications.Analytical and bioanalytical chemistry · 2022Article
- Knowledge gaps in late-onset neonatal sepsis in preterm neonates: a roadmap for future research.Pediatric research · 2022Review
- Platelets and Immature Neutrophils in Preterm Infants with Feeding Intolerance.American journal of perinatology · 2021Article
- In Vitro and Vivo Identification, Metabolism and Action of Xenoestrogens: An Overview.International journal of molecular sciences · 2021Review
- Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities.Nano letters · 2020Article
- Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6.Sensors (Basel, Switzerland) · 2020Review
- Point of care technologies for sepsis diagnosis and treatment.Lab on a chip · 2019Review
- Precision Medicine for Neonatal Sepsis.Frontiers in molecular biosciences · 2018Review
- A Chip for Estrogen Receptor Action: Detection of Biomarkers Released by MCF-7 Cells through Estrogenic and Anti-Estrogenic Effects.Sensors (Basel, Switzerland) · 2017Article
- Analytical Protein Microarrays: Advancements Towards Clinical Applications.Sensors (Basel, Switzerland) · 2017Review
- ELISA in the multiplex era: potentials and pitfalls.Proteomics. Clinical applications · 2015Review
- Academic College of Emergency Experts in India's INDO-US Joint Working Group and OPUS12 Foundation Consensus Statement on Creating A Coordinated, Multi-Disciplinary, Patient-Centered, Global Point-of-Care Biomarker Discovery Network.International journal of critical illness and injury science · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neonatal sepsis is still a leading cause of death among newborns. Therefore a protein-microarray for point-of-care testing that simultaneously quantifies the sepsis associated serum proteins IL-6, IL-8, IL-10, TNF alpha, S-100, PCT, E-Selectin, CRP and Neopterin has been developed. The chip works with only a 4 μL patient serum sample and hence minimizes excessive blood withdrawal from newborns. The 4 μL patient samples are diluted with 36 μL assay buffer and distributed to four slides for repetitive measurements. Streptavidin coated magnetic particles that act as distinct stirring detection components are added, not only to stir the sample, but also to detect antibody antigen binding events. We demonstrate that the test is complete within 2.5 h using a single step assay. S-100 conjugated to BSA is spotted in increasing concentrations to create an internal calibration. The presented low volume protein-chip fulfills the requirements of point-of-care testing for accurate and repeatable (CV < 14%) quantification of serum proteins for the diagnosis of neonatal sepsis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.