ArticleJournal of virology2012
Effects of manipulating apoptosis on Sindbis virus infection of Aedes aegypti mosquitoes.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 77 citations in OpenAlex.
- Host serum-circulating flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense.Nature communications · 2026Article
- Temporal and spatial profiling of Aedes albopictus immune responses to chikungunya virus infection.PLoS neglected tropical diseases · 2025Article
- From perception to action: MAPKs in plant-virus interactions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Beet curly top virus affects vector biology: the first transcriptome analysis of the beet leafhopper.The Journal of general virology · 2024Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- Factors Affecting Arbovirus Midgut Escape in Mosquitoes.Pathogens (Basel, Switzerland) · 2023Review
- The host transcriptome change involved in the inhibitory effect of exogenous interferon-γ on Getah virus replication.Frontiers in microbiology · 2023Article
- The Role of Mosquito Hemocytes in Viral Infections.Viruses · 2022Review
- Article
- PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.Nature communications · 2022Article
- Advances in the Immune Regulatory Role of Non-Coding RNAs (miRNAs and lncRNAs) in Insect-Pathogen Interactions.Frontiers in immunology · 2022Review
- Article
- Activation of the Host Immune Response inInsects · 2021Article
- Engorgement of Rhipicephalus haemaphysaloides ticks blocked by silencing a protein inhibitor of apoptosis.Experimental & applied acarology · 2021Article
- Clustered rapid induction of apoptosis limits ZIKV and DENV-2 proliferation in the midguts of Aedes aegypti.Communications biology · 2021Article
- Review
- A Hyperactive Kunjin Virus NS3 Helicase Mutant Demonstrates Increased Dissemination and Mortality in Mosquitoes.Journal of virology · 2020Article
- Effects of Manipulating Fibroblast Growth Factor Expression on Sindbis Virus Replication In Vitro and inViruses · 2020Article
- Article
- RNA Interference Is Enhanced by Knockdown of double-stranded RNases in the Yellow Fever MosquitoInsects · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improved control of vector-borne diseases requires an understanding of the molecular factors that determine vector competence. Apoptosis has been shown to play a role in defense against viruses in insects and mammals. Although some observations suggest a correlation between apoptosis and resistance to arboviruses in mosquitoes, there is no direct evidence tying apoptosis to arbovirus vector competence. To determine whether apoptosis can influence arbovirus replication in mosquitoes, we manipulated apoptosis in Aedes aegypti mosquitoes by silencing the expression of genes that either positively or negatively regulate apoptosis. Silencing of the A. aegypti anti-apoptotic gene iap1 (Aeiap1) caused apoptosis in midgut epithelium, alterations in midgut morphology, and 60 to 70% mosquito mortality. Mortality induced by Aeiap1 silencing was rescued by cosilencing the initiator caspase gene Aedronc, indicating that the mortality was due to apoptosis. When mosquitoes which had been injected with Aeiap1 double-stranded RNA (dsRNA) were orally infected with Sindbis virus (SINV), increased midgut infection and virus dissemination to other organs were observed. This increase in virus infection may have been due to the effects of widespread apoptosis on infection barriers or innate immunity. In contrast, silencing the expression of Aedronc, which would be expected to inhibit apoptosis, reduced SINV midgut infection and virus dissemination. Thus, our data suggest that some level of caspase activity and/or apoptosis may be necessary for efficient virus replication and dissemination in mosquitoes. This is the first study to directly test the roles of apoptosis and caspases in determining mosquito vector competence for arboviruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.