ArticleJournal of virology2012
The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- Transferrin receptor 1 is required for efficient hepatitis E virus production.PLoS pathogens · 2026Article
- Integrated Bioinformatics Analysis Reveals the Impact of SHEV ORF3-Related LncRNA Network on Bile Secretion Pathway (ko 04976) in HepG2 Cells.Veterinary sciences · 2026Article
- Identification of Riboflavin Metabolism Pathway in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3 Protein.Veterinary sciences · 2025Article
- Cell line-dependent release of quasi-enveloped hepatitis E virus reveals alternative Golgi-associated egress in the absence of pORF3.Frontiers in cellular and infection microbiology · 2025Article
- Characteristics and Functions of HEV Proteins.Advances in experimental medicine and biology · 2023Article
- Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus.Veterinary sciences · 2022Article
- Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.Microbiology spectrum · 2022Article
- Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada.Viruses · 2021Article
- Open reading frame 3 protein of hepatitis E virus: Multi-function protein with endless potential.World journal of gastroenterology · 2021Review
- Deep Insight Into Long Non-coding RNA and mRNA Transcriptome Profiling in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3.Frontiers in veterinary science · 2021Article
- A novel avian isolate of hepatitis E virus from Pakistan.Virology journal · 2019Article
- Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein.Journal of virology · 2019Article
- Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion.PLoS pathogens · 2018Article
- Life cycle and morphogenesis of the hepatitis E virus.Emerging microbes & infections · 2018Review
- Vaccine Development against Zoonotic Hepatitis E Virus: Open Questions and Remaining Challenges.Frontiers in microbiology · 2018Review
- Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus.Viruses · 2016Article
- Role of Envelopment in the HEV Life Cycle.Viruses · 2016Review
- Molecular Biology and Infection of Hepatitis E Virus.Frontiers in microbiology · 2016Review
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The ORF3 protein of hepatitis E virus (HEV) is a multifunctional protein important for virus replication. The ORF3 proteins from human, swine, and avian strains of HEV contain a conserved PXXP amino acid motif, resembling either Src homology 3 (SH3) cell signaling interaction motifs or "late domains" involved in host cell interactions aiding in particle release. Using an avian strain of HEV, we determined the roles of the conserved prolines within the PREPSAPP motif in HEV replication and infectivity in Leghorn male hepatoma (LMH) chicken liver cells and in chickens. Each proline was changed to alanine to produce 8 avian HEV mutants containing single mutations (P64, P67, P70, and P71 to A), double mutations (P64/67A, P64/70A, and P67/70A), and triple mutations (P64/67/70A). The results showed that avian HEV mutants are replication competent in vitro, and none of the prolines in the PXXPXXPP motif are essential for infectivity in vivo; however, the second and third prolines appear to aid in fecal virus shedding, suggesting that the PSAP motif, but not the PREP motif, is involved in virus release. We also showed that the PSAP motif interacts with the host protein tumor suppressor gene 101 (TSG101) and that altering any proline within the PSAP motif disrupts this interaction. However, we showed that the ORF2 protein expressed in LMH cells is efficiently released from the cells in the absence of ORF3 and that coexpression of ORF2 and ORF3 did not act synergistically in this release, suggesting that another factor(s) such as ORF1 or viral genomic RNA may be necessary for proper particle release.
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