ArticleThe Journal of biological chemistry2012
Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Autoinhibition and activation mechanisms revealed by the triangular-shaped structure of myosin Va.Science advances · 2022Article
- Autoregulation and dual stepping mode of MYA2, an Arabidopsis myosin XI responsible for cytoplasmic streaming.Scientific reports · 2022Article
- Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines.The EMBO journal · 2022Article
- Effects of Mutations in the Phenamacril-Binding Site ofACS omega · 2020Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Creation of a myosin Va-TAP-tagged mouse and identification of potential myosin Va-interacting proteins in the cerebellum.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Regulation of class V myosin.Cellular and molecular life sciences : CMLS · 2018Review
- Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Globular Tail Domain of Myosin-5a Functions as a Dimer in Regulating the Motor Activity.The Journal of biological chemistry · 2016Article
- Identification of the Isoform-specific Interactions between the Tail and the Head of Class V Myosin.The Journal of biological chemistry · 2016Article
- Melanophilin Stimulates Myosin-5a Motor Function by Allosterically Inhibiting the Interaction between the Head and Tail of Myosin-5a.Scientific reports · 2015Article
- Mouse myosin-19 is a plus-end-directed, high-duty ratio molecular motor.The Journal of biological chemistry · 2014Article
- Surface dynamics in allosteric regulation of protein-protein interactions: modulation of calmodulin functions by Ca2+.PLoS computational biology · 2013Article
- Structural insights into the globular tails of the human type v myosins Myo5a, Myo5b, And Myo5c.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myosin 5a is as yet the best-characterized unconventional myosin motor involved in transport of organelles along actin filaments. It is well-established that myosin 5a is regulated by its tail in a Ca(2+)-dependent manner. The fact that the actin-activated ATPase activity of myosin 5a is stimulated by micromolar concentrations of Ca(2+) and that calmodulin (CaM) binds to IQ motifs of the myosin 5a heavy chain indicates that Ca(2+) regulates myosin 5a function via bound CaM. However, it is not known which IQ motif and bound CaM are responsible for the Ca(2+)-dependent regulation and how the head-tail interaction is affected by Ca(2+). Here, we found that the CaM in the first IQ motif (IQ1) is responsible for Ca(2+) regulation of myosin 5a. In addition, we demonstrate that the C-lobe fragment of CaM in IQ1 is necessary for mediating Ca(2+) regulation of myosin 5a, suggesting that the C-lobe fragment of CaM in IQ1 participates in the interaction between the head and the tail. We propose that Ca(2+) induces a conformational change of the C-lobe of CaM in IQ1 and prevents interaction between the head and the tail, thus activating motor function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.