ArticleThe Journal of biological chemistry2012
Structural characterization of closely related O-antigen lipopolysaccharide (LPS) chain length regulators.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Chain-Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis.Molecular microbiology · 2026Article
- Isolation and characterization of a broad-spectrumFrontiers in microbiology · 2026Article
- Structural Basis of Lipopolysaccharide O-Antigen Chain Length Modality.Research (Washington, D.C.) · 2026Article
- Recent insights into Wzy polymerases and lipopolysaccharide O-antigen biosynthesis.Journal of bacteriology · 2025Review
- Repeat-Unit Elongations To Produce Bacterial Complex Long Polysaccharide Chains, an O-Antigen Perspective.EcoSal Plus · 2023Review
- Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE.Communications biology · 2023Article
- Identification of the Shigella flexneri Wzy Domain Modulating WzzJournal of bacteriology · 2022Article
- Structure of a full-length bacterial polysaccharide co-polymerase.Nature communications · 2021Article
- Review
- Unique Regions of the Polysaccharide Copolymerase WzzJournal of bacteriology · 2019Article
- Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.International journal of microbiology · 2018Review
- Synthesis of Rhizobial Exopolysaccharides and Their Importance for Symbiosis with Legume Plants.Genes · 2017Review
- Quaternary structure of WzzB and WzzE polysaccharide copolymerases.Protein science : a publication of the Protein Society · 2015Article
- Challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways.Frontiers in microbiology · 2015Review
- Article
- Conserved-residue mutations in Wzy affect O-antigen polymerization and Wzz-mediated chain-length regulation in Pseudomonas aeruginosa PAO1.Scientific reports · 2013Article
- The D3 bacteriophage α-polymerase inhibitor (Iap) peptide disrupts O-antigen biosynthesis through mimicry of the chain length regulator Wzz in Pseudomonas aeruginosa.Journal of bacteriology · 2013Article
- Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.Trends in microbiology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The surface O-antigen polymers of gram-negative bacteria exhibit a modal length distribution that depends on dedicated chain length regulator periplasmic proteins (polysaccharide co-polymerases, PCPs) anchored in the inner membrane by two transmembrane helices. In an attempt to determine whether structural changes underlie the O-antigen modal length specification, we have determined the crystal structures of several closely related PCPs, namely two chimeric PCP-1 family members solved at 1.6 and 2.8 Å and a wild-type PCP-1 from Shigella flexneri solved at 2.8 Å. The chimeric proteins form circular octamers, whereas the wild-type WzzB from S. flexneri was found to be an open trimer. We also present the structure of a Wzz(FepE) mutant, which exhibits severe attenuation in its ability to produce very long O-antigen polymers. Our findings suggest that the differences in the modal length distribution depend primarily on the surface-exposed amino acids in specific regions rather than on the differences in the oligomeric state of the PCP protomers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.