Evidence map›Paper›PMID 22437535›Full record

ArticleJournal of veterinary science2012

Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea.

Dong-Kun Yang, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song

Abstract readComparative Study
In one paragraph

Article in Journal of veterinary science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Review
  6. Validation of laboratory tests for infectious diseases in wild mammals: review and recommendations.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Rabies Epidemiology and Control in Ecuador.Global journal of health science · 2015
    Observational
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dong-Kun YangAnimal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea. yangdk@korea.kr
Eun-Kyung Shin
Yoon-I Oh
Kyung-Woo Lee
Chung-San Lee
Seo-Young Kim
Jeong-A Lee
Jae-Young Song

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100, and 95%, respectively. The specificities of VI, RT-PCR and RIDA were found to be 100, 100, and 98.9%, respectively. Rabies viruses circulating in Korea were isolated and propagated in murine neuroblastoma (NG108-15) cells with titers ranging from 10(1.5) to 10(4.5) TCID(50)/mL. Although the RIDA findings did not completely coincide with results obtained from FAT, VI, and RT-PCR, RIDA appears to be a fast and reliable assay that can be used to analyze brain samples. In summary, the results from our study showed that VI, RT-PCR, and RIDA can be used as supplementary diagnostic tools for detecting rabies viruses in both laboratory and field settings.

Indexed as

AnimalsAntigens, ViralBrainFluorescent Antibody Technique, IndirectImmunoassayRabiesRabies virusRepublic of KoreaReverse Transcriptase Polymerase Chain ReactionRNA, ViralSensitivity and SpecificityAntigens, ViralRNA, Viral

Identifiers

PMID22437535
PMCPMC3317456

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.