ArticleBMC biotechnology2012
The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.Nature biotechnology · 2026Article
- High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration.Science advances · 2023Article
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Genetic switches designed for eukaryotic cells and controlled by serine integrases.Communications biology · 2020Article
- A memory switch for plant synthetic biology based on the phage ϕC31 integration system.Nucleic acids research · 2020Article
- Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector.Genes · 2019Article
- Development of a high efficiency integration system and promoter library for rapid modification ofMetabolic engineering communications · 2017Article
- Multiple expression cassette exchange via TP901-1, R4, and Bxb1 integrase systems on a mouse artificial chromosome.FEBS open bio · 2017Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Serine integrase chimeras with activity in E. coli and HeLa cells.Biology open · 2014Article
- Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase.BMC biotechnology · 2014Article
- Molecular Genetics of Mycobacteriophages.Microbiology spectrum · 2014Article
- Cross-talk between diverse serine integrases.Journal of molecular biology · 2014Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and mammalian cells.
resultsIn this work, the Bxb1 recombinase gene was transformed and constitutively expressed in Arabidopsis thaliana plants harboring a chromosomally integrated attP and attB-flanked target sequence. The Bxb1 recombinase successfully excised the target sequence in a conservative manner and the resulting recombination event was heritably transmitted to subsequent generations in the absence of the recombinase transgene. In addition, we also show that Bxb1 recombinase expressing plants can be manually crossed with att-flanked target transgenic plants to generate excised progeny.
conclusionThe Bxb1 large serine recombinase performs site-specific recombination in Arabidopsis thaliana germinal tissue, producing stable lines free of unwanted DNA. The precise site-specific deletion produced by Bxb1 in planta demonstrates that this enzyme can be a useful tool for the genetic engineering of plants without selectable marker transgenes or other undesirable exogenous sequences.
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