ArticleVirology journal2012
Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host.
Article in Virology journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
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- Large-genome phage vB_Eco_ZCEC15 targets gastrointestinal MDR E. coli: evidence from in vitro and Caco-2 cell models.BMC microbiology · 2026Article
- Identification and analysis of the crucial holin domain and sites and the bactericidal activity of a holin-endolysin lysis cassette from phage PZL-Ah152 againstJournal of virology · 2026Article
- Unveiling the antibacterial effects of endolysin POE and holin Hol8 from Pseudomonas otitidis phage vB_PotS-PotUPM1.World journal of microbiology & biotechnology · 2026Article
- Genomic characterization of novel lytic phage vB_Sal_S6 with putative host FhuA interaction and its application for Salmonella biocontrol in milk.Scientific reports · 2025Article
- Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii.BMC microbiology · 2025Article
- Reclassification of the first Bacillus tropicus phage calls for reclassification of other Bacillus temperate phages previously designated as plasmids.BMC genomics · 2024Article
- Genomic Characterization of Phage ZP3 and Its Endolysin LysZP with Antimicrobial Potential againstViruses · 2024Article
- ComI inhibits transformation inJournal of bacteriology · 2024Article
- Efficient Recovery of Complete Gut Viral Genomes by Combined Short- and Long-Read Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Archaeal virus entry and egress.microLife · 2024Review
- Characterization and genomic analysis of a novel bacteriophage BUCT_49532 lysing Klebsiella pneumoniae.Virus genes · 2023Article
- The Lytic Activity of Bacteriophage ZCSE9 againstViruses · 2023Article
- Isolation of bacteriophages infectingFrontiers in microbiology · 2023Article
- Prediction and identification of new type holin protein ofFood science and biotechnology · 2022Article
- Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications.Frontiers in microbiology · 2022Review
- Characterization and Genomic Analysis of a Novel Drexlervirial Bacteriophage IME268 with Lytic Activity AgainstInfection and drug resistance · 2022Article
- Bacteriophage Proteome: Insights and Potentials of an Alternate to Antibiotics.Infectious diseases and therapy · 2021Review
- Isolation and Characterization of Novel Phages TargetingPHAGE (New Rochelle, N.Y.) · 2021Article
- Characterization and Full Genome Sequence of Novel KPP-5 Lytic Phage againstBiomedicines · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHolins are a group of phage-encoded membrane proteins that control access of phage-encoded endolysins to the peptidoglycan, and thereby trigger the lysis process at a precise time point as the 'lysis clock'. SMP is an isolated and characterized Streptococcus suis lytic phage. The aims of this study were to determine the holin gene, HolSMP, in the genome of SMP, and characterized the function of holin, HolSMP, in phage infection.
resultsHolSMP was predicted to encode a small membrane protein with three hydrophobic transmembrane helices. During SMP infections, HolSMP was transcribed as a late gene and HolSMP accumulated harmlessly in the cell membrane before host cell lysis. Expression of HolSMP in Escherichia coli induced an increase in cytoplasmic membrane permeability, an inhibition of host cell growth and significant cell lysis in the presence of LySMP, the endolysin of phage SMP. HolSMP was prematurely triggered by the addition of energy poison to the medium. HolSMP complemented the defective λ S allele in a non-suppressing Escherichia coli strain to produce phage plaques.
conclusionsOur results suggest that HolSMP is the holin protein of phage SMP and a two-step lysis system exists in SMP.
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