ArticleMolecular microbiology2012
Diversity in prokaryotic glycosylation: an archaeal-derived N-linked glycan contains legionaminic acid.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- S-layer architecture and glycosylation and their role in shaping virus-host specificity in prokaryotes.Frontiers in microbiology · 2026Review
- Glycoproteins in Viruses.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Article
- Perturbed N-glycosylation of Halobacterium salinarum archaellum filaments leads to filament bundling and compromised cell motility.Nature communications · 2024Article
- N-glycosylation in Archaea - Expanding the process, components and roles of a universal post-translational modification.BBA advances · 2024Article
- Article
- Article
- Discovery and Characterization of Thermoproteus Spherical Piliferous Virus 1: a Spherical Archaeal Virus Decorated with Unusual Filaments.Journal of virology · 2020Article
- MYXO-CTERM sorting tag directs proteins to the cell surface via the type II secretion system.Molecular microbiology · 2020Article
- CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection.Nature communications · 2019Article
- Article
- The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins.Nature communications · 2019Article
- Archaeal cell surface biogenesis.FEMS microbiology reviews · 2018Article
- Genomic variation and biogeography of Antarctic haloarchaea.Microbiome · 2018Article
- Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the HalophileFrontiers in microbiology · 2018Article
- Exploration of the Sialic Acid World.Advances in carbohydrate chemistry and biochemistry · 2018Review
- Emerging facets of prokaryotic glycosylation.FEMS microbiology reviews · 2017Review
- AglM and VNG1048G, Two Haloarchaeal UDP-Glucose Dehydrogenases, Show Different Salt-Related Behaviors.Life (Basel, Switzerland) · 2016Article
- Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids.Journal of virology · 2015Article
- Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi.BMC genomics · 2015Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
VP4, the major structural protein of the haloarchaeal pleomorphic virus, HRPV-1, is glycosylated. To define the glycan structure attached to this protein, oligosaccharides released by β-elimination were analysed by mass spectrometry and nuclear magnetic resonance spectroscopy. Such analyses showed that the major VP4-derived glycan is a pentasaccharide comprising glucose, glucuronic acid, mannose, sulphated glucuronic acid and a terminal 5-N-formyl-legionaminic acid residue. This is the first observation of legionaminic acid, a sialic acid-like sugar, in an archaeal-derived glycan structure. The importance of this residue for viral infection was demonstrated upon incubation with N-acetylneuraminic acid, a similar monosaccharide. Such treatment reduced progeny virus production by half 4 h post infection. LC-ESI/MS analysis confirmed the presence of pentasaccharide precursors on two different VP4-derived peptides bearing the N-glycosylation signal, NTT. The same sites modified by the native host, Halorubrum sp. strain PV6, were also recognized by the Haloferax volcanii N-glycosylation apparatus, as determined by LC-ESI/MS of heterologously expressed VP4. Here, however, the N-linked pentasaccharide was the same as shown to decorate the S-layer glycoprotein in this species. Hence, N-glycosylation of the haloarchaeal viral protein, VP4, is host-specific. These results thus present additional examples of archaeal N-glycosylation diversity and show the ability of Archaea to modify heterologously expressed proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.