ArticleTissue engineering. Part C, Methods2012
Longitudinal in vivo imaging to assess blood flow and oxygenation in implantable engineered tissues.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Topological Tracks Patterned via 3D Printing Vascularize Murine Organ-Scale Constructs.Cell biomaterials · 2026Article
- Controlled oxygen delivery to power tissue regeneration.Nature communications · 2024Review
- Vascularized 3D Human Skin Models in the Forefront of Dermatological Research.Advanced healthcare materials · 2024Review
- Scanning darkfield high-resolution microendoscope for label-free microvascular imaging.Biomedical optics express · 2023Article
- Engineered tissue vascularization and engraftment depends on host model.Scientific reports · 2023Article
- Engineering the multiscale complexity of vascular networks.Nature reviews. Materials · 2022Review
- Tissues with Patterned Vessels or Protein Release Induce Vascular Chemotaxis in anTissue engineering. Part A · 2021Article
- Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct SuccessTissue engineering. Part C, Methods · 2021Article
- Mature vessel networks in engineered tissue promote graft-host anastomosis and prevent graft thrombosis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Wearable speckle plethysmography (SPG) for characterizing microvascular flow and resistance.Biomedical optics express · 2018Article
- Noninvasive Optical Assessment of Implanted Engineered Tissues Correlates with Cytokine Secretion.Tissue engineering. Part C, Methods · 2018Article
- Correcting for motion artifact in handheld laser speckle images.Journal of biomedical optics · 2018Article
- Momentum transfer Monte Carlo for the simulation of laser speckle imaging and its application in the skin.Biomedical optics express · 2017Article
- Prevascularized silicon membranes for the enhancement of transport to implanted medical devices.Journal of biomedical materials research. Part B, Applied biomaterials · 2016Article
- Design and evaluation of a miniature laser speckle imaging device to assess gingival health.Journal of biomedical optics · 2016Article
- Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering.Acta biomaterialia · 2016Article
- Acute discrimination between superficial-partial and deep-partial thickness burns in a preclinical model with laser speckle imaging.Burns : journal of the International Society for Burn Injuries · 2015Article
- Recent developments in vascular imaging techniques in tissue engineering and regenerative medicine.BioMed research international · 2015Review
- Implanted cell-dense prevascularized tissues develop functional vasculature that supports reoxygenation after thrombosis.Tissue engineering. Part A · 2014Article
- A three-dimensional in vitro model of tumor cell intravasation.Integrative biology : quantitative biosciences from nano to macro · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The functionality of vascular networks within implanted prevascularized tissues is difficult to assess using traditional analysis techniques, such as histology. This is largely due to the inability to visualize hemodynamics in vivo longitudinally. Therefore, we have developed dynamic imaging methods to measure blood flow and hemoglobin oxygen saturation in implanted prevascularized tissues noninvasively and longitudinally. Using laser speckle imaging, multispectral imaging, and intravital microscopy, we demonstrate that fibrin-based tissue implants anastomose with the host (severe combined immunodeficient mice) in as short as 20 h. Anastomosis results in initial perfusion with highly oxygenated blood, and an increase in average hemoglobin oxygenation of 53%. However, shear rates in the preformed vessels were low (20.8±12.8 s(-1)), and flow did not persist in the vast majority of preformed vessels due to thrombus formation. These findings suggest that designing an appropriate vascular network structure in prevascularized tissues to maintain shear rates above the threshold for thrombosis may be necessary to maintain flow following implantation. We conclude that wide-field and microscopic functional imaging can dynamically assess blood flow and oxygenation in vivo in prevascularized tissues, and can be used to rapidly evaluate and improve prevascularization strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.