ArticleProtein science : a publication of the Protein Society2012
The protein gp74 from the bacteriophage HK97 functions as a HNH endonuclease.
Article in Protein science : a publication of the Protein Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 33 citations in OpenAlex.
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- Functional Evaluation of a Conserved HNH Endonuclease Gene in Lactococcal Skunavirus Genomes.Microbial biotechnology · 2026Article
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- Whole Genome Sequence Analysis ofPolish journal of microbiology · 2022Article
- ArsR Family Regulator MSMEG_6762 Mediates the Programmed Cell Death by Regulating the Expression of HNH Nuclease in Mycobacteria.Microorganisms · 2022Article
- First Characterization of aFrontiers in microbiology · 2021Article
- HK97 gp74 Possesses an α-Helical Insertion in the ββα Fold That Affects Its Metal Binding,Journal of bacteriology · 2020Article
- A protein architecture guided screen for modification dependent restriction endonucleases.Nucleic acids research · 2019Article
- Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic CyanobacteriumApplied and environmental microbiology · 2019Article
- Staphylococcus sciuri bacteriophages double-convert for staphylokinase and phospholipase, mediate interspecies plasmid transduction, and package mecA gene.Scientific reports · 2017Article
- Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.Current microbiology · 2016Article
- Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.Scientific reports · 2016Article
- Ultrastructure and Viral Metagenome of Bacteriophages from an Anaerobic Methane OxidizingFrontiers in microbiology · 2016Article
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- Comparative Genomics of theFrontiers in microbiology · 2016Article
- The Protein Interactome of Mycobacteriophage Giles Predicts Functions for Unknown Proteins.Journal of bacteriology · 2015Article
- Genome Analysis of Phage SMSAP5 as Candidate of Biocontrol for Staphylococcus aureus.Korean journal for food science of animal resources · 2015Article
- Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.Nucleic acids research · 2014Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
The last gene in the genome of the bacteriophage HK97 encodes the protein gp74. We present data in this article that demonstrates, for the first time, that gp74 possesses HNH endonuclease activity. HNH endonucleases are small DNA binding and digestion proteins characterized by two His residues and an Asn residue. We demonstrate that gp74 cleaves lambda phage DNA at multiple sites and that gp74 requires divalent metals for its endonuclease activity. We also present intrinsic tryptophan fluorescence data that show direct binding of Ni(2+) to gp74. The activity of gp74 in the presence of Ni(2+) is significantly decreased below neutral pH, suggesting the presence of one or more His residues in metal binding and/or DNA digestion. Surprisingly, this pH-dependence of activity is not seen with Zn(2+) , suggesting a different mode of binding of Zn(2+) and Ni(2+) . This difference in activity may result from binding of a second Zn(2+) ion by a putative zinc finger in gp74 in addition to binding of a Zn(2+) ion by the HNH motif. These studies define the biochemical function of gp74 as an HNH endonuclease and provide a platform for determining the role of gp74 in life cycle of the bacteriophage HK97.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.