ArticleNeurochemical research2012
Efficient gene transfer into neonatal mouse brain using electroporation.
Article in Neurochemical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- A reliable and flexible gene manipulation strategy in posthatch zebra finch brain.Scientific reports · 2017Article
- Rapid and efficient gene delivery into the adult mouse brain via focal electroporation.Scientific reports · 2016Article
- Specific expression of foreign gene in postnatal mouse glial cells using electroporation.CNS neuroscience & therapeutics · 2014Article
- Gene transfer into the both sides of postnatal cerebral cortex using tweezer electrode.CNS neuroscience & therapeutics · 2013Article
- Harnessing DNA-induced immune responses for improving cancer vaccines.Human vaccines & immunotherapeutics · 2012Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo electroporation works as an effective method to transfer exogenous genes into postnatal rodent forebrain. Nevertheless, two deficiencies were found in the reported methods. First, surgical operation brings unnecessary trauma to newborn pups. Second, the procedure was complicated and the transfection efficiency was relatively low. Here we improved the previous electroporation method and make it more simple and efficient. The pulse voltage was decreased to 90 v. DNA injection into one pup's forebrain could be completed within 30 s without any surgical operation. More than 94% of injected neonates survived. Almost 100% of the survivors expressed the introduced gene and the expression persists as long as 20 days after injection. Thus, this method offers a powerful new way for gene function study in postnatal neurogenesis and neural development.
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Registered trials
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