ArticlePlant physiology2012
Serine/threonine/tyrosine protein kinase phosphorylates oleosin, a regulator of lipid metabolic functions.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Epigenetic Alterations in Microbiome-Host Interactions in Inflammatory and Autoimmune Diseases.International journal of molecular sciences · 2026Review
- Identification of metabolism-related subtypes and feature genes of pre-eclampsia.Scientific reports · 2025Article
- Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.BMC plant biology · 2024Article
- Exploiting lipid droplet metabolic pathway to foster lipid production: oleosin in focus.Plant cell reports · 2024Review
- Identification of a Major QTL for Seed Protein Content in Cultivated Peanut (Plants (Basel, Switzerland) · 2024Article
- Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers.International journal of molecular sciences · 2024Article
- Molecular Machinery of Lipid Droplet Degradation and Turnover in Plants.International journal of molecular sciences · 2023Review
- Structural and Functional Annotation of Napin-Like Protein from Momordica charantia to Explore its Medicinal Importance.Biochemical genetics · 2022Article
- How plants solubilise seed fats: revisiting oleosin structure and function to inform commercial applications.Biophysical reviews · 2022Review
- Plant Kinases in the Perception and Signaling Networks Associated With Arthropod Herbivory.Frontiers in plant science · 2022Review
- Linkage and association analyses reveal that hub genes in energy-flow and lipid biosynthesis pathways form a cluster in upland cotton.Computational and structural biotechnology journal · 2022Article
- Genome-wide identification and analysis of Oleosin gene family in four cotton species and its involvement in oil accumulation and germination.BMC plant biology · 2021Article
- Article
- Range-wide neutral and adaptive genetic structure of an endemic herb from Amazonian Savannas.AoB PLANTS · 2020Article
- A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana is Involved in Plant Growth and Abiotic Stress Response.Plants (Basel, Switzerland) · 2020Article
- Biochemical characterization of Plasmodium falciparum parasite specific helicase 1 (PfPSH1).FEBS open bio · 2019Article
- Artificial selection on GmOLEO1 contributes to the increase in seed oil during soybean domestication.PLoS genetics · 2019Article
- Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination inPlant diversity · 2018Article
- Arabidopsis serine/threonine/tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds.Scientific reports · 2018Article
- Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenicBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant oils are stored in oleosomes or oil bodies, which are surrounded by a monolayer of phospholipids embedded with oleosin proteins that stabilize the structure. Recently, a structural protein, Oleosin3 (OLE3), was shown to exhibit both monoacylglycerol acyltransferase and phospholipase A(2) activities. The regulation of these distinct dual activities in a single protein is unclear. Here, we report that a serine/threonine/tyrosine protein kinase phosphorylates oleosin. Using bimolecular fluorescence complementation analysis, we demonstrate that this kinase interacts with OLE3 and that the fluorescence was associated with chloroplasts. Oleosin-green fluorescent protein fusion protein was exclusively associated with the chloroplasts. Phosphorylated OLE3 exhibited reduced monoacylglycerol acyltransferase and increased phospholipase A(2) activities. Moreover, phosphatidylcholine and diacylglycerol activated oleosin phosphorylation, whereas lysophosphatidylcholine, oleic acid, and Ca(2+) inhibited phosphorylation. In addition, recombinant peanut (Arachis hypogaea) kinase was determined to predominantly phosphorylate serine residues, specifically serine-18 in OLE3. Phosphorylation levels of OLE3 during seed germination were determined to be higher than in developing peanut seeds. These findings provide direct evidence for the in vivo substrate selectivity of the dual-specificity kinase and demonstrate that the bifunctional activities of oleosin are regulated by phosphorylation.
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