ArticlePloS one2012
Functional and molecular characterization of rod-like cells from retinal stem cells derived from the adult ciliary epithelium.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Increasing cell culture density during a developmental window prevents fated rod precursors derailment toward hybrid rod-glia cells.Scientific reports · 2023Article
- Depleted Calcium Stores and Increased Calcium Entry in Rod Photoreceptors of theInternational journal of molecular sciences · 2022Article
- Activation of adult mammalian retinal stem cells in vivo via antagonism of BMP and sFRP2.Stem cell research & therapy · 2021Article
- Review
- New In Vitro Cellular Model for Molecular Studies of Retinitis Pigmentosa.International journal of molecular sciences · 2021Article
- Glucocorticoid agonists enhance retinal stem cell self-renewal and proliferation.Stem cell research & therapy · 2021Article
- Targeting molecular pathways for the treatment of inherited retinal degeneration.Neural regeneration research · 2020Review
- Hydroxyindole-Heliyon · 2019Article
- Novel hybrid action of GABA mediates inhibitory feedback in the mammalian retina.PLoS biology · 2019Article
- Adult Stem Cells, Tools for Repairing the Retina.Current ophthalmology reports · 2019Article
- Combination of cGMP analogue and drug delivery system provides functional protection in hereditary retinal degeneration.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.Stem cell reports · 2015Article
- Photoreceptor cells with profound structural deficits can support useful vision in mice.Investigative ophthalmology & visual science · 2014Article
- Gene expression-based comparison of the human secretory neuroepithelia of the brain choroid plexus and the ocular ciliary body: potential implications for glaucoma.Fluids and barriers of the CNS · 2014Article
- Adult ciliary epithelial stem cells generate functional neurons and differentiate into both early and late born retinal neurons under non-cell autonomous influences.BMC neuroscience · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In vitro generation of photoreceptors from stem cells is of great interest for the development of regenerative medicine approaches for patients affected by retinal degeneration and for high throughput drug screens for these diseases. In this study, we show unprecedented high percentages of rod-fated cells from retinal stem cells of the adult ciliary epithelium. Molecular characterization of rod-like cells demonstrates that they lose ciliary epithelial characteristics but acquire photoreceptor features. Rod maturation was evaluated at two levels: gene expression and electrophysiological functionality. Here we present a strong correlation between phototransduction protein expression and functionality of the cells in vitro. We demonstrate that in vitro generated rod-like cells express cGMP-gated channels that are gated by endogenous cGMP. We also identified voltage-gated channels necessary for rod maturation and viability. This level of analysis for the first time provides evidence that adult retinal stem cells can generate highly homogeneous rod-fated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.