ArticlePloS one2012
Disinfection of ocular cells and tissues by atmospheric-pressure cold plasma.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 133 citations in OpenAlex.
- Feasibility and Safety of Argon Cold Plasma Use as an Adjunctive Treatment for Corneal Disease in Dogs, Cats and Small Mammals: A Prospective Clinical Study.Veterinary ophthalmology · 2026Article
- Treatment and Cleaning of Contact Lenses with Plasma-Activated Solutions.Pathogens (Basel, Switzerland) · 2026Article
- The effects of ex vivo physical cold atmospheric plasma treatment on endothelial cells in human corneal explants and its potential for in vivo application in refractory corneal ulcers.Scientific reports · 2025Article
- Treatment of Clinically Important Bacteria With Cold Atmospheric Plasma.Microbial biotechnology · 2025Review
- Biomedical application of cold plasma: navigating through plasma types and devices by antimicrobial effectiveness and tissue tolerance.Advanced therapeutics · 2025Article
- Immunological Control of Herpes Simplex Virus Type 1 Infection: A Non-Thermal Plasma-Based Approach.Viruses · 2025Review
- Modified protocol comparing sporicidal activity of different non-thermal plasma generating devices.Scientific reports · 2025Article
- Disinfection of Human and Porcine Corneal Endothelial Cells by Far-UVC Irradiation.Medicina (Kaunas, Lithuania) · 2025Article
- Short-term effects of argon cold atmospheric plasma on canine corneasFrontiers in veterinary science · 2025Article
- Candidate SNP Markers Significantly Altering the Affinity of the TATA-Binding Protein for the Promoters of Human Genes Associated with Primary Open-Angle Glaucoma.International journal of molecular sciences · 2024Article
- Mechanism of lipid peroxidation of liposomes by cold atmospheric pressure plasma jet irradiation.Journal of clinical biochemistry and nutrition · 2024Article
- How does atmospheric pressure cold helium plasma affect the biomechanical behaviour on alkali-lesioned corneas?BMC veterinary research · 2024Article
- Non-Thermal Plasma Reduces HSV-1 Infection of and Replication in HaCaT Keratinocytes In Vitro.International journal of molecular sciences · 2024Article
- Direct Applications of Non-Thermal Atmospheric Pressure Plasma: An Emerging Therapeutic Era in Ophthalmology.Clinical ophthalmology (Auckland, N.Z.) · 2024Review
- Two separate mechanisms are involved in membrane permeabilization during lipid oxidation.Biophysical journal · 2023Article
- Innovative cold atmospheric plasma (iCAP) decreases corneal ulcer formation and bacterial loads and improves anterior chamber health in methicillin resistant Staphylococcus aureus keratitis.Experimental eye research · 2023Article
- Optimization of Anthocyanin Production in Tobacco Cells.International journal of molecular sciences · 2023Article
- Manipulation of Oxidative Stress Responses by Non-Thermal Plasma to Treat Herpes Simplex Virus Type 1 Infection and Disease.International journal of molecular sciences · 2023Review
- Argon cold atmospheric plasma eradicates pathogensFrontiers in veterinary science · 2023Article
- Treatment of Fresh Meat, Fish and Products Thereof with Cold Atmospheric Plasma to Inactivate Microbial Pathogens and Extend Shelf Life.Foods (Basel, Switzerland) · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLow temperature plasmas have been proposed in medicine as agents for tissue disinfection and have received increasing attention due to the frequency of bacterial resistance to antibiotics. This study explored whether atmospheric-pressure cold plasma (APCP) generated by a new portable device that ionizes a flow of helium gas can inactivate ocular pathogens without causing significant tissue damage. METHODOLOGY/PRINCIPAL
findingsWe tested the APCP effects on cultured Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, Aspergillus fumigatus and Herpes simplex virus-1, ocular cells (conjunctival fibroblasts and keratocytes) and ex-vivo corneas. Exposure to APCP for 0.5 to 5 minutes significantly reduced microbial viability (colony-forming units) but not human cell viability (MTT assay, FACS and Tunel analysis) or the number of HSV-1 plaque-forming units. Increased levels of intracellular reactive oxygen species (ROS) in exposed microorganisms and cells were found using a FACS-activated 2',7'-dichlorofluorescein diacetate probe. Immunoassays demonstrated no induction of thymine dimers in cell cultures and corneal tissues. A transient increased expression of 8-OHdG, genes and proteins related to oxidative stress (OGG1, GPX, NFE2L2), was determined in ocular cells and corneas by HPLC, qRT-PCR and Western blot analysis.
conclusionsA short application of APCP appears to be an efficient and rapid ocular disinfectant for bacteria and fungi without significant damage on ocular cells and tissues, although the treatment of conjunctival fibroblasts and keratocytes caused a time-restricted generation of intracellular ROS and oxidative stress-related responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.