ArticlePloS one2012
Identification of the first functional toxin-antitoxin system in Streptomyces.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Characterization of Two Toxin-Antitoxin Systems in Deep-SeaMarine drugs · 2019Article
- Article
- The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production inFrontiers in microbiology · 2018Article
- Development of an antibiotic marker-free platform for heterologous protein production in Streptomyces.Microbial cell factories · 2017Article
- Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488.Scientific reports · 2016Article
- Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems.Frontiers in molecular biosciences · 2016Review
- Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.Scientific reports · 2015Article
- YoeB toxin is activated during thermal stress.MicrobiologyOpen · 2015Article
- The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid pSLE1 and linear plasmid pSLE2.BMC genomics · 2015Article
- Die for the community: an overview of programmed cell death in bacteria.Cell death & disease · 2015Review
- Draft Genome Sequence of Streptomyces fradiae ATCC 19609, a Strain Highly Sensitive to Antibiotics.Genome announcements · 2014Article
- The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation.The Biochemical journal · 2014Article
- Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production.Applied and environmental microbiology · 2014Article
- Light activation of Staphylococcus aureus toxin YoeBSa1 reveals guanosine-specific endoribonuclease activity.Biochemistry · 2014Article
- YoeB-ribosome structure: a canonical RNase that requires the ribosome for its specific activity.Nucleic acids research · 2013Article
- Pre-sporulation stages of Streptomyces differentiation: state-of-the-art and future perspectives.FEMS microbiology letters · 2013Review
- Stable expression plasmids for Streptomyces based on a toxin-antitoxin system.Microbial cell factories · 2013Article
- The axe-txe complex of Enterococcus faecium presents a multilayered mode of toxin-antitoxin gene expression regulation.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxin-antitoxin (TA) systems are widespread among the plasmids and genomes of bacteria and archaea. This work reports the first description of a functional TA system in Streptomyces that is identical in two species routinely used in the laboratory: Streptomyces lividans and S. coelicolor. The described system belongs to the YefM/YoeB family and has a considerable similarity to Escherichia coli YefM/YoeB (about 53% identity and 73% similarity). Lethal effect of the S. lividans putative toxin (YoeBsl) was observed when expressed alone in E. coli SC36 (MG1655 ΔyefM-yoeB). However, no toxicity was obtained when co-expression of the antitoxin and toxin (YefM/YoeBsl) was carried out. The toxic effect was also observed when the yoeBsl was cloned in multicopy in the wild-type S. lividans or in a single copy in a S. lividans mutant, in which this TA system had been deleted. The S. lividans YefM/YoeBsl complex, purified from E. coli, binds with high affinity to its own promoter region but not to other three random selected promoters from Streptomyces. In vivo experiments demonstrated that the expression of yoeBsl in E. coli blocks translation initiation processing mRNA at three bases downstream of the initiation codon after 2 minutes of induction. These results indicate that the mechanism of action is identical to that of YoeB from E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.