ArticlePloS one2012
PhOTO zebrafish: a transgenic resource for in vivo lineage tracing during development and regeneration.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Optimizing photoactivation of PA-mCherry for optical pooled CRISPR screens.FEBS open bio · 2026Article
- Cells adapt to extracellular acidic pH through TM9SF3-mediated PI(4,5)PNature communications · 2025Article
- Zebrafish as a Versatile Model for Cardiovascular Research: Peering into the Heart of the Matter.Cells · 2025Review
- Nervous system-wide analysis of allScience advances · 2025Article
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- Reduced sister chromatid cohesion acts as a tumor penetrance modifier.PLoS genetics · 2022Article
- Biodegradable Harmonophores for Targeted High-ResolutionACS nano · 2021Article
- Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches.Bio-protocol · 2020Article
- Functional Heterogeneity within the Developing Zebrafish Epicardium.Developmental cell · 2020Article
- Article
- Optimization of a 2A self-cleaving peptide-based multigene expression system for efficient expression of upstream and downstream genes in silkworm.Molecular genetics and genomics : MGG · 2019Article
- Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo.Journal of visualized experiments : JoVE · 2018Article
- Animal models of fibrodysplasia ossificans progressiva.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Review
- The rise of photoresponsive protein technologies applicationsF1000Research · 2017Review
- Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.Nature protocols · 2016Article
- Determination of the source of SHG verniers in zebrafish skeletal muscle.Scientific reports · 2015Article
- 2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyx mori.Scientific reports · 2015Article
- Applications of phototransformable fluorescent proteins for tracking the dynamics of cellular components.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Review
- Article
- Advances in whole-embryo imaging: a quantitative transition is underway.Nature reviews. Molecular cell biology · 2014Review
Corrections and comments
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundElucidating the complex cell dynamics (divisions, movement, morphological changes, etc.) underlying embryonic development and adult tissue regeneration requires an efficient means to track cells with high fidelity in space and time. To satisfy this criterion, we developed a transgenic zebrafish line, called PhOTO, that allows photoconvertible optical tracking of nuclear and membrane dynamics in vivo. METHODOLOGY: PhOTO zebrafish ubiquitously express targeted blue fluorescent protein (FP) Cerulean and photoconvertible FP Dendra2 fusions, allowing for instantaneous, precise targeting and tracking of any number of cells using Dendra2 photoconversion while simultaneously monitoring global cell behavior and morphology. Expression persists through adulthood, making the PhOTO zebrafish an excellent tool for studying tissue regeneration: after tail fin amputation and photoconversion of a ∼100 µm stripe along the cut area, marked differences seen in how cells contribute to the new tissue give detailed insight into the dynamic process of regeneration. Photoconverted cells that contributed to the regenerate were separated into three distinct populations corresponding to the extent of cell division 7 days after amputation, and a subset of cells that divided the least were organized into an evenly spaced, linear orientation along the length of the newly regenerating fin. CONCLUSIONS/SIGNIFICANCE: PhOTO zebrafish have wide applicability for lineage tracing at the systems-level in the early embryo as well as in the adult, making them ideal candidate tools for future research in development, traumatic injury and regeneration, cancer progression, and stem cell behavior.
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