ArticlePloS one2012
Development and functional analysis of novel genetic promoters using DNA shuffling, hybridization and a combination thereof.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
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- De novo transcriptome profiling revealing genes involved in piperine biosynthetic pathway in Piper longum L.Scientific reports · 2025Article
- Recent advances in designing synthetic plant regulatory modules.Frontiers in plant science · 2025Review
- Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses.Frontiers in plant science · 2024Article
- Analysis of plant pararetrovirus promoter sequence(s) for developing a useful synthetic promoter with enhanced activity in rice, pearl millet, and tobacco plants.Frontiers in plant science · 2024Article
- Bacterial bioreporters for the detection of trace explosives: performance enhancement by DNA shuffling and random mutagenesis.Applied microbiology and biotechnology · 2021Article
- Article
- Synthetic promoters in planta.Planta · 2015Review
- Utilization of plant-derived recombinant human β-defensins (hBD-1 and hBD-2) for averting salmonellosis.Transgenic research · 2015Article
- Panspecies small-molecule disruptors of heterochromatin-mediated transcriptional gene silencing.Molecular and cellular biology · 2015Article
- Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4) exhibits growth inhibitory effects in prostate cancer cells.Frontiers in plant science · 2015Article
- Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.Nature protocols · 2014Article
- Efficient chimeric plant promoters derived from plant infecting viral promoter sequences.Planta · 2014Article
- pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDevelopment of novel synthetic promoters with enhanced regulatory activity is of great value for a diverse range of plant biotechnology applications. METHODOLOGY: Using the Figwort mosaic virus full-length transcript promoter (F) and the sub-genomic transcript promoter (FS) sequences, we generated two single shuffled promoter libraries (LssF and LssFS), two multiple shuffled promoter libraries (LmsFS-F and LmsF-FS), two hybrid promoters (FuasFScp and FSuasFcp) and two hybrid-shuffled promoter libraries (LhsFuasFScp and LhsFSuasFcp). Transient expression activities of approximately 50 shuffled promoter clones from each of these libraries were assayed in tobacco (Nicotiana tabacum cv. Xanthi) protoplasts. It was observed that most of the shuffled promoters showed reduced activity compared to the two parent promoters (F and FS) and the CaMV35S promoter. In silico studies (computer simulated analyses) revealed that the reduced promoter activities of the shuffled promoters could be due to their higher helical stability. On the contrary, the hybrid promoters FuasFScp and FSuasFcp showed enhanced activities compared to F, FS and CaMV 35S in both transient and transgenic Nicotiana tabacum and Arabidopsis plants. Northern-blot and qRT-PCR data revealed a positive correlation between transcription and enzymatic activity in transgenic tobacco plants expressing hybrid promoters. Histochemical/X-gluc staining of whole transgenic seedlings/tissue-sections and fluorescence images of ImaGene Green™ treated roots and stems expressing the GUS reporter gene under the control of the FuasFScp and FSuasFcp promoters also support the above findings. Furthermore, protein extracts made from protoplasts expressing the human defensin (HNP-1) gene driven by hybrid promoters showed enhanced antibacterial activity compared to the CaMV35S promoter. SIGNIFICANCE/
conclusionBoth shuffled and hybrid promoters developed in the present study can be used as molecular tools to study the regulation of ectopic gene expression in plants.
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