ArticleJournal of biomedicine & biotechnology2012
Potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme.
Article in Journal of biomedicine & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme.Stem cell reviews and reports · 2019Trial
- In vitro culture-induced pluripotency of human spermatogonial stem cells.BioMed research international · 2013Trial
- Sertoli cell-only syndrome: advances, challenges, and perspectives in genetics and mechanisms.Cellular and molecular life sciences : CMLS · 2023Review
- The Effect of Normal Follicular Fluid on the Differentiation of PCOS Ovarian Stem Cells into Oocyte-Like Cells.Advanced biomedical research · 2023Article
- A homozygous PIWIL2 frameshift variant affects the formation and maintenance of human-induced pluripotent stem cell-derived spermatogonial stem cells and causes Sertoli cell-only syndrome.Stem cell research & therapy · 2022Article
- Endogenous, tissue-resident stem/progenitor cells in gonads and bone marrow express FSHR and respond to FSH via FSHR-3.Journal of ovarian research · 2021Review
- Additional Evidence to Establish Existence of Two Stem Cell Populations Including VSELs and SSCs in Adult Mouse Testes.Stem cell reviews and reports · 2020Article
- Pluripotent Very Small Embryonic-Like Stem Cells in Adult Testes - An Alternate Premise to Explain Testicular Germ Cell Tumors.Stem cell reviews and reports · 2018Article
- Stem cells survive oncotherapy & can regenerate non-functional gonads: A paradigm shift for oncofertility.The Indian journal of medical research · 2018Review
- Cryostorage of immature and mature human testis tissue to preserve spermatogonial stem cells (SSCs): a systematic review of current experiences toward clinical applications.Stem cells and cloning : advances and applications · 2018Review
- Making gametes from alternate sources of stem cells: past, present and future.Reproductive biology and endocrinology : RB&E · 2017Review
- Underlying Mechanisms that Restore Spermatogenesis on Transplanting Healthy Niche Cells in Busulphan Treated Mouse Testis.Stem cell reviews and reports · 2016Article
- Making gametes from pluripotent stem cells: embryonic stem cells or very small embryonic-like stem cells?Stem cell investigation · 2016Article
- Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells.Stem cells international · 2016Article
- Putative mesenchymal stem cells isolated from adult human ovaries.Journal of assisted reproduction and genetics · 2014Article
- Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women.BioMed research international · 2014Article
- Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture.BioMed research international · 2014Article
- Isolation and characterization of novel, highly proliferative human CD34/CD73-double-positive testis-derived stem cells for cell therapy.Stem cells and development · 2013Article
- Small SSEA-4-positive cells from human ovarian cell cultures: related to embryonic stem cells and germinal lineage?Journal of ovarian research · 2013Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal- and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.