ArticleThe Journal of biological chemistry2012
Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS.Nucleic acids research · 2024Article
- Review
- Review
- Archaeal DNA Repair Mechanisms.Biomolecules · 2020Review
- New Insights Into DNA Repair Revealed by NucS Endonucleases From Hyperthermophilic Archaea.Frontiers in microbiology · 2020Review
- Endonucleases responsible for DNA repair of helix-distorting DNA lesions in the thermophilic crenarchaeon Sulfolobus acidocaldarius in vivo.Extremophiles : life under extreme conditions · 2019Article
- Activation of the mismatch-specific endonuclease EndoMS/NucS by the replication clamp is required for high fidelity DNA replication.Nucleic acids research · 2018Article
- Physical and functional interplay between PCNA DNA clamp and Mre11-Rad50 complex from the archaeon Pyrococcus furiosus.Nucleic acids research · 2018Article
- Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.Nucleic acids research · 2017Article
- PCNA-binding proteins in the archaea: novel functionality beyond the conserved core.Current genetics · 2016Review
- Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea.Nucleic acids research · 2016Article
- Dynamics of the heme-binding bacterial gas-sensing dissimilative nitrate respiration regulator (DNR) and activation barriers for ligand binding and escape.The Journal of biological chemistry · 2014Article
- Differential interaction kinetics of a bipolar structure-specific endonuclease with DNA flaps revealed by single-molecule imaging.PloS one · 2014Article
- Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication.Nucleic acids research · 2013Article
- Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrococcus abyssi NucS is the founding member of a new family of structure-specific DNA endonucleases that interact with the replication clamp proliferating cell nuclear antigen (PCNA). Using a combination of small angle x-ray scattering and surface plasmon resonance analyses, we demonstrate the formation of a stable complex in solution, in which one molecule of the PabNucS homodimer binds to the outside surface of the PabPCNA homotrimer. Using fluorescent labels, PCNA is shown to increase the binding affinity of NucS toward single-strand/double-strand junctions on 5' and 3' flaps, as well as to modulate the cleavage specificity on the branched DNA structures. Our results indicate that the presence of a single major contact between the PabNucS and PabPCNA proteins, together with the complex-induced DNA bending, facilitate conformational flexibility required for specific cleavage at the single-strand/double-strand DNA junction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.