ArticleThe Journal of biological chemistry2012
LHCBM1 and LHCBM2/7 polypeptides, components of major LHCII complex, have distinct functional roles in photosynthetic antenna system of Chlamydomonas reinhardtii.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 92 citations in OpenAlex.
- Cross-species transcriptomics and transgenic analyses implicate major LHCII genes in microalgal responses to ammonium stress.Frontiers in plant science · 2026Article
- Transcriptional gene fusions via targeted integration at safe harbors for high transgene expression in Chlamydomonas reinhardtii.The New phytologist · 2025Article
- Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas.Plant physiology · 2024Article
- The role of the pigment-protein complex LHCBM1 in nonphotochemical quenching in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Dynamic Regulation of the Light-Harvesting System through State Transitions in Land Plants and Green Algae.Plants (Basel, Switzerland) · 2023Review
- Plastoquinone pool redox state and control of state transitions in Chlamydomonas reinhardtii in darkness and under illumination.Photosynthesis research · 2023Article
- LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2021Article
- Structural basis of LhcbM5-mediated state transitions in green algae.Nature plants · 2021Article
- Light Harvesting in Fluctuating Environments: Evolution and Function of Antenna Proteins across Photosynthetic Lineage.Plants (Basel, Switzerland) · 2021Article
- Heterologous expression of cyanobacterial Orange Carotenoid Protein (OCP2) as a soluble carrier of ketocarotenoids inAlgal research · 2021Article
- Structure of photosystem I-LHCI-LHCII from the green alga Chlamydomonas reinhardtii in State 2.Nature communications · 2021Article
- Conformational Dynamics of Light-Harvesting Complex II in a Native Membrane Environment.Biophysical journal · 2021Article
- Chlamydomonas reinhardtii LHCSR1 and LHCSR3 proteins involved in photoprotective non-photochemical quenching have different quenching efficiency and different carotenoid affinity.Scientific reports · 2020Article
- Regulation of Light Harvesting inPlant physiology · 2020Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana.Photosynthesis research · 2019Article
- Structural insight into light harvesting for photosystem II in green algae.Nature plants · 2019Article
- Four distinct trimeric forms of light-harvesting complex II isolated from the green alga Chlamydomonas reinhardtii.Photosynthesis research · 2019Article
- miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii.ACS synthetic biology · 2019Article
- Photoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms.The Journal of biological chemistry · 2018Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The photosystem II antenna of Chlamydomonas reinhardtii is composed of monomeric and trimeric complexes, the latter encoded by LHCBM genes. We employed artificial microRNA technology to specifically silence the LHCBM2 and LHCBM7 genes, encoding identical mature polypeptides, and the LHCBM1 gene. As a control, we studied the npq5 mutant, deficient in the LHCBM1 protein. The organization of LHCII complexes, functional antenna size, capacity for photoprotection, thermal energy dissipation and state transitions, and resistance to reactive oxygen species was studied in the various genotypes. Silencing of the LHCBM2/7 genes resulted in a decrease of an LHCII protein with an apparent molecular mass of 22 kDa, whereas silencing/lack of LHCBM1 caused the decrease/disappearance of a 23-kDa protein. A decrease in the abundance of trimeric LHCII complexes and in functional antenna size was observed in both LHCBM2/7 and LHCBM1 knockouts. In agreement with previous data, depletion of LHCBM1 decreased the capacity for excess energy dissipation but not the ability to perform state transitions. The opposite was true for LHCBM2/7, implying that this polypeptide has a different functional role from LHCBM1. The abundance of LHCBM1 and LHCBM2/7 is in both cases correlated with resistance to superoxide anion, whereas only LHCBM1 is also involved in singlet oxygen scavenging. These results suggest that different LHCBM components have well defined, non-redundant functions despite their high homology, implying that engineering of LHCBM proteins can be an effective strategy for manipulating the light harvesting system of Chlamydomonas reinhardtii.
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