ArticleProceedings of the National Academy of Sciences of the United States of America2012
Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 88 citations in OpenAlex.
- SPIFFI enables single-shot super-resolution and multidimensional imaging.Nature methods · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.Nanophotonics (Berlin, Germany) · 2025Article
- Examination of conformational dynamics of AdiC transporter with fluorescence-polarization microscopy.The Journal of general physiology · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Article
- Correlative imaging of the spatio-angular dynamics of biological systems with multimodal instant polarization microscope.Biomedical optics express · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Measuring 3D orientation of nanocrystals via polarized luminescence of rare-earth dopants.Nature communications · 2021Article
- POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy.Advances in experimental medicine and biology · 2021Article
- Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy.Frontiers in chemistry · 2020Article
- Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences.Computational and structural biotechnology journal · 2020Review
- Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.Nature communications · 2019Article
- Polarized super-resolution structural imaging inside amyloid fibrils using Thioflavine T.Scientific reports · 2017Article
- Seeing the unseen: Imaging rotation in cells with designer anisotropic particles.Micron (Oxford, England : 1993) · 2017Review
- Monitoring the orientation of rare-earth-doped nanorods for flow shear tomography.Nature nanotechnology · 2017Article
- Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Reconstructing 3D deformation dynamics for curved epithelial sheet morphogenesis from positional data of sparsely-labeled cells.Nature communications · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule fluorescence polarization techniques have been used for three-dimensional (3D) orientation measurements to observe the dynamic properties of single molecules. However, only few techniques can simultaneously measure 3D orientation and position. Furthermore, these techniques often require complex equipment and cumbersome analysis. We have developed a microscopy system and synthesized highly fluorescent, rod-like shaped quantum dots (Q rods), which have linear polarizations, to simultaneously measure the position and 3D orientation of a single fluorescent probe. The optics splits the fluorescence from the probe into four different spots depending on the polarization angle and projects them onto a CCD camera. These spots are used to determine the 2D position and 3D orientation. Q rod orientations could be determined with better than 10° accuracy at 33 ms time resolution. We applied our microscopy and Q rods to simultaneously measure myosin V movement along an actin filament and rotation around its own axis, finding that myosin V rotates 90° for each step. From this result, we suggest that in the two-headed bound state, myosin V necks are perpendicular to one another, while in the one-headed bound state the detached trailing myosin V head is biased forward in part by rotating its lever arm about its own axis. This microscopy system should be applicable to a wide range of dynamic biological processes that depend on single molecule orientation dynamics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.