ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1990
Long-term maintenance of primary myogenic cultures on a reconstituted basement membrane.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 40 citations in OpenAlex.
- Available In Vitro Models for Human Satellite Cells from Skeletal Muscle.International journal of molecular sciences · 2021Review
- The Satellite Cell at 60: The Foundation Years.Journal of neuromuscular diseases · 2021Review
- In Vitro Innervation as an Experimental Model to Study the Expression and Functions of Acetylcholinesterase and Agrin in Human Skeletal Muscle.Molecules (Basel, Switzerland) · 2017Review
- Differentiation of human gingival mesenchymal stem cells into neuronal lineages in 3D bioconjugated injectable protein hydrogel construct for the management of neuronal disorder.Experimental & molecular medicine · 2016Article
- Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.Tissue engineering. Part C, Methods · 2014Article
- Skeletal muscle satellite cells: background and methods for isolation and analysis in a primary culture system.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion.Journal of cell science · 2006Article
- Development and postnatal regulation of adult myoblasts.Microscopy research and technique · 1995Review
- Electrical activity-dependent regulation of the acetylcholine receptor delta-subunit gene, MyoD, and myogenin in primary myotubes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Skeletal muscle satellite cells appear during late chicken embryogenesis.Developmental biology · 1992Article
- Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture.Differentiation; research in biological diversityArticle
- Myogenesis in the Chicken: the Onset of Differentiation of Adult Myoblasts is Influenced by Tissue Factors.Basic and applied myology : BAMArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We describe a simple technique for maintaining highly contractile long-term chicken myogenic cultures on Matrigel, a gel composed of basement membrane components extracted from the Engelbreth-Holm-Swarm mouse tumor. Cultures grown on Matrigel consist of three-dimensional multilayers of cylindrical, contracting myotubes which endure for at least 60 d without myotube detachment. A Matrigel substrate increases the initial plating efficiency but does not effect cell proliferation. Large-scale differentiation in cultures maintained on Matrigel is delayed by 1 to 2 d, compared to cultures grown on gelatin-coated dishes. Long-term maintenance on Matrigel also results in increased expression of the neonatal and adult fast myosin heavy chain isoforms. Culturing of cells on a Matrigel substrate could thus facilitate the study of later events of in vitro myogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.