ArticleBMC veterinary research2012
Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri.
Article in BMC veterinary research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Engineering human endometrial model systems in reproductive health and disease.npj biomedical innovations · 2025Review
- Luminal and Glandular Epithelial Cells from the Porcine Endometrium maintain Cell Type-Specific Marker Gene Expression in Air-Liquid Interface Culture.Stem cell reviews and reports · 2022Article
- Methodology for the establishment of primary porcine vocal fold epithelial cell cultures.The Laryngoscope · 2019Article
- Bovine Endometrial Epithelial Cells Scale Their Pro-inflammatory ResponseFrontiers in cellular and infection microbiology · 2017Article
- Effects of Female Sex Hormones on Susceptibility to HSV-2 in Vaginal Cells Grown in Air-Liquid Interface.Viruses · 2016Article
- Article
- Innate immunity in the vagina (part I): estradiol inhibits HBD2 and elafin secretion by human vaginal epithelial cells.American journal of reproductive immunology (New York, N.Y. : 1989) · 2013Article
- Air-liquid and liquid-liquid interfaces influence the formation of the urothelial permeability barrier in vitro.In vitro cellular & developmental biology. Animal · 2013Article
- Engineered hypopharynx from coculture of epithelial cells and fibroblasts using poly(ester urethane) as substratum.BioMed research international · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo-like properties of the tissue.
resultsWe tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. We could reproducibly culture pure epithelial cells from cervical tissue explants. Based on a morphology score and the WST-1 Proliferation Assay, we optimized the growth medium composition. Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. After cultivation in an air-liquid interface for three weeks, the cells showed a discontinuously multilayered phenotype. Finally, differentiation was validated via immunohistochemistry against beta catenin. Mucopolysaccharide production could be shown via alcian blue staining.
conclusionsWe provide the first suitable protocol to establish a differentiated porcine epithelial model of the cervix uteri, based on easily accessible cells using slaughterhouse material.
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