ArticleActa ophthalmologica2013
Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan.
Article in Acta ophthalmologica, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- DNA Oxidation and Expression of Repair Enzymes in Organ- Cultured Human Limbal Epithelium.International journal of molecular sciences · 2026Article
- Article
- Influence of Organ Culture on the Characteristics of the Human Limbal Stem Cell Niche.International journal of molecular sciences · 2023Article
- Epithelial Regeneration in Human Corneas Preserved in an Active Storage Machine.Translational vision science & technology · 2021Article
- Article
- Ocular Cell Lines and Genotoxicity Assessment.International journal of environmental research and public health · 2020Review
- Levels of oxidative DNA damage are low in ex vivo engineered human limbal epithelial tissue.Acta ophthalmologica · 2018Article
- Effect of different culture media and deswelling agents on survival of human corneal endothelial and epithelial cells in vitro.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2016Article
- Epithelial cells as alternative human biomatrices for comet assay.Frontiers in genetics · 2014Review
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Authors and funding
10 authors.
Funding
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Abstract
purposeStorage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC.
methodsMorphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT-PCR and changes in oxidative lipid and DNA damage by ELISA and COMET assay.
resultsA prominent loss of cells was observed after storage in Optisol GS. After maintenance in EBOC, spreading apical cells were Occludin(+) , while the staining for E-cadherin and Connexin-43 was less intense. There were an upregulation of Occludin and a downregulation of E-cadherin and Connexin-43. Eye Bank Organ Culture was associated with an ongoing proliferative activity and a downregulation of putative progenitor/stem cell marker ABCG2 and p63. Staining for 8-OHdG and Caspase-3 did not increase, while levels of malondialdehyde and number of DNA strand breaks and oxidized bases increased.
conclusionsThis dual procedure should be pursued as an option to increase the storage time and the pool of available donor corneas. The observed downregulation of markers associated with stemness during EBOC is relevant considering the potential use of donor epithelium in the treatment of ocular surface disorders.
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