ArticleJournal of the American Chemical Society2012
Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Core polymer optimization of ternary siRNA nanoparticles enhances in vivo safety, pharmacokinetics, and tumor gene silencing.Biomaterials · 2023Article
- Incorporation of a FRET Pair into a Riboswitch RNA to Measure MgInternational journal of molecular sciences · 2022Article
- Regulation of E. coli Rep helicase activity by PriC.Journal of molecular biology · 2021Article
- Dynamics of the context-specific translation arrest by chloramphenicol and linezolid.Nature chemical biology · 2020Article
- Single-Molecule Fluorescence Applied to Translation.Cold Spring Harbor perspectives in biology · 2019Review
- Article
- 2'-O-methylation in mRNA disrupts tRNA decoding during translation elongation.Nature structural & molecular biology · 2018Article
- Three tRNAs on the ribosome slow translation elongation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling.Cell reports · 2017Article
- Sonophore labeled RGD: a targeted contrast agent for optoacoustic imaging.Photoacoustics · 2017Article
- Amino acid sequence repertoire of the bacterial proteome and the occurrence of untranslatable sequences.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Direct correlation of DNA binding and single protein domain motion via dual illumination fluorescence microscopy.Nano letters · 2014Article
- smFRET studies of the 'encounter' complexes and subsequent intermediate states that regulate the selectivity of ligand binding.FEBS letters · 2014Review
- Dynamic pathways of -1 translational frameshifting.Nature · 2014Article
- Signal recognition particle-ribosome binding is sensitive to nascent chain length.The Journal of biological chemistry · 2014Article
- Sequence-dependent elongation dynamics on macrolide-bound ribosomes.Cell reports · 2014Article
- The dynamics of SecM-induced translational stalling.Cell reports · 2014Article
- High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Frameshifting dynamics.Biopolymers · 2013Review
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4 authors.
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Abstract
Single-molecule Förster resonance energy transfer (smFRET) is a powerful method for studying the conformational dynamics of a biomolecule in real-time. However, studying how interacting ligands correlate with and regulate the conformational dynamics of the biomolecule is extremely challenging because of the availability of a limited number of fluorescent dyes with both high quantum yield and minimal spectral overlap. Here we report the use of a nonfluorescent quencher (Black Hole Quencher, BHQ) as an acceptor for smFRET. Using a Cy3/BHQ pair, we can accurately follow conformational changes of the ribosome during elongation in real time. We demonstrate the application of single-color FRET to correlate the conformational dynamics of the ribosome with the compositional dynamics of tRNA. We use the normal Cy5 FRET acceptor to observe arrival of a fluorescently labeled tRNA with a concomitant transition of the ribosome from the locked to the unlocked conformation. Our results illustrate the potential of nonfluorescent quenchers in single-molecule correlation studies.
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